补精益视片对大鼠慢性高眼压模型视网膜损害的影响

目的　观察补精益视片对大鼠慢性高眼压（ｅｌｅｖａｔｅｄｉｎｔｒａｏｃｕｌａｒｐｒｅｓｓｕｒｅ，ＥＩＯＰ）模型视网膜损害的干预作用，探讨其作用机理。方法　将３０只ＳＤ大鼠随机分为３组:对照组、给药组、模型组，每组１０只。采用烙闭上巩膜静脉法对给药组、模型组ＳＤ大鼠建立慢性ＥＩＯＰ模型，观察补精益视片对慢性ＥＩＯＰ大鼠眼压和视网膜病理形态学变化的影响。结果　给药组、模型组大鼠在造模后即刻直至造模后８周与造模前相比眼压均升高，差异均有统计学意义（均为Ｐ＜０．０１），说明ＥＩＯＰ模型造模成功。造模后８周眼压与造模后即刻相比，给药组有降低趋势，差异有统计学意义（Ｐ＜０．０１），模型组差异无统计学意义（Ｐ＞０．０５）。造模后８周，视网膜神经节细胞（ｒｅｔｉｎａｌｇａｎｇｌｉｏｎｃｅｌｌｓ，ＲＧＣｓ）数量给药组（１４．５７±１．９７）与模型组（１０．７６±２．１９）均明显低于对照组（１７．４７±１．９７），差异均有统计学意义（Ｐ＜０．０５，Ｐ＜０．０１）；视网膜厚度模型组为（１５０．８３±１７．９１）μｍ低于对照组的（２１９．７２±３２．２４）μｍ，差异有统计学意义（Ｐ＜０．０１），给药组为（２１５．５１±５１．２３）μｍ，与对照组相比差异无统计学意义（Ｐ＞０．０５）；给药组ＲＧＣｓ数量及视网膜厚度均优于模型组，差异均有统计学意义（均为Ｐ＜０．０１）。ＲＧＣｓ超微结构显示给药组较模型组明显改善。结论　补精益视片能保护ＳＤ大鼠慢性ＥＩＯＰ模型视功能，表现为降低眼压、提高ＲＧＣｓ数量、增加视网膜厚度、改善ＲＧＣｓ超微结构。

Effects of Bujingyishi tablet on retinal damage in rat model of chronic elevated intraocular pressure

Objective To observe the effects of bujingyishi tablet on the retinal damage in rat model of elevated intraocular pressure ( EIOP) . and prelinunarily explore its mechanism. Methods Thirty SD rats were randomly and equally divided int0 3 groups : control group , model group and treatment group , 10 rats in each group. By unilaterally cauterizing three episcleral vessels . the rat model of chronic EIOP was established on model group and treatment group. The effects of bujingyishi tablet on intraocular pressure ( IOP) and pathological changes of retina were observed. Results Compared with pre-operation,IOP in treatment group and model group at models building until postoperative 8 weeks increased ( all P < 0. 01 ) . indicated that the EIOP models were successfully established. There was obviously reduce in treatment group between postoperation 8 weeks and models buiding ( P < 0. 01 ) . while model group was not statistically sigruficant ( P > 0. 05 ) . At postoperative 8 weeks. the number of retinal ganglion cells( RGCs) in treatment group( 14. 57 + 1. 97 ) and model group ( 10. 76 + 2. 19 ) were lower than that in control group ( 17. 47 + 1. 97 ) ( P < 0. 05 .P < 0. 01 ) . The retinal thickness had statistically sigruficant difference between control group ( 219. 72 + 32. 24 ) ym and model group ( 150. 83 + 17. 91 ) ym ( P < 0. 01 ) , While there was not statistically significant between control group and treatment group ( 215. 5 I + 51. 23 ) ym ( P > 0. 05 ) . The number of RGCs and retinal thickness in treatment group were better than those in model group ( all P < 0. 01) . The improvement of RGCs ultrastructure in treatment group was better than that in model group. Conclusion Bujingyishi tablet can protect the visual function in the rat model of chronic EIOP,can decrease IOP.increase the number of RGCs and retinal thickness ,improve the ultrastructure of RGCs.