食管鳞癌中IDO的表达及其与肿瘤血管形成的关系

目的 检测吲哚胺2,3-双加氧酶（Indoleamine 2,3-dioxygenase, IDO）在人食管鳞癌及其癌旁组织中的表达及规律，通过检测VEGF的表达探索IDO及肿瘤血管形成的相关性，并了解两者在食管鳞癌的进展及转移中的作用。方法 收集56例河北医科大学第四医院行食管切除患者的肿瘤组织及其癌旁组织，使用半定量RT-PCR法和免疫组织化学法检测组织样本中IDO和VEGF的mRNA和蛋白表达情况，并分析在食管鳞癌组织中IDO与肿瘤血管形成的相关性。结果 与癌旁组织相比，食管鳞癌组织中的IDO和VEGF阳性表达率和表达水平均显著升高（P=0.020，P=0.022），其表达与患者年龄、性别等无关（P＞0.05），与患者TNM分期、肿瘤侵犯深度、分化程度、淋巴结转移相关（P＜0.05）。食管鳞癌组织中IDO的表达与VEGF的表达呈显著正相关（r=0.533，P=0.001）。结论 在食管鳞癌中IDO可能与肿瘤血管的形成有关。

IDO Expression in Esophageal Squamous Cell Carcinoma and Its Relationship with Tumor Angiogenesis

Objective To test the expression of IDO and VEGF mRNA in tumor tissues and para-carcinoma tissues of esophageal squamous cell carcinoma(ESCC)patients and to investigate their associations with tumor invasion and lymphatic metastasis. Methods Carcinoma tissue and para-carcinoma tissue specimens were collected from 56 cases of ESCC patients who underwent esophagectomy in The Fourth Hospital of Hebei Medical University. Optical density semi-quantitative RT-PCR and immunohistochemical method were used to detect the expression of IDO and VEGF mRNA. The association between the expressions of IDO, VEGF mRNA and patients’ clinical features were analyzed. Results Compared with para-carcinoma tissues, the expression levels of IDO and VEGF mRNA in ESCC tissues were significantly increased (P=0.020, P=0.022). The expression of IDO and VEGF mRNA were both associated with patients’ TNM stage, tumor invasion depth, tumor differentiation and lymph node metastasis (P＜0.05), but age or gender(P＞0.05). Meanwhile the expression of IDO mRNA was positively correlated with VEGF mRNA significantly (r=0.533, P=0.001). Conclusion In ESCC tissues, the expression of IDO and VEGF mRNA are significantly up-regulated. These changes of gene expressions are associated with patients’ clinical features, which indicate that they might play a key role in tumor invasion and lymph node metastasis.