| 丙酮酸乙酯对百草枯中毒小鼠肺损伤的保护作用 |
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| 引用本文： | 邵阳,罗治彬,高洁,何建川,刘世平,游箭.丙酮酸乙酯对百草枯中毒小鼠肺损伤的保护作用[J].实用医学杂志,2010,26(7). |
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| 作者姓名： | 邵阳 罗治彬 高洁 何建川 刘世平 游箭 |
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| 作者单位： | 1. 川北医学院附属医院,四川省南充市,637000
2. 第三军医大学野战外科研究所,创伤、烧伤与复合伤国家重点实验室,重庆市,400042 |
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| 基金项目： | 四川省杰出青年学科带头人培养基金资助项目，四川省科技攻关项目 |
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| 摘 要： | 目的:探讨百草枯(paraquat)时小鼠肺损伤的作用机制,及丙酮酸乙酯对肺损伤的保护作用.方法:C57BL 小鼠腹腔注射百草枯(20mg/kg)诱导损伤(PI组);2h后腹腔注射丙酮酸乙酯(40 mg/kg).在损伤6、12、24 h后,酶联免疫吸附法(ELIS)检测血清血浆肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)水平;24 h后观察肺病理改变;免疫组织化学检测Caspase-3的蛋白表达.结果:PI组小鼠血清TNF-α和IL-1β水平在给予百草枯后迅速升高,约在6 h达高峰,之后迅速下降.经丙酮酸乙酯治疗,血清TNF-α和IL-1β水平均低于PI组,且在6h差异显著(P<0.01).结论:百草枯可引起小鼠细胞炎性因子TNF-α和IL-1β大量产生,TNF-α和IL-1β可能参与肺损伤过程;早期给予丙酮酸乙酯能降低TNF-α和IL-1β的释放,降低caspase-3蛋白的表达,抑制细胞凋亡,可以减轻肺组织损伤.
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| 关 键 词： | 肺疾病 百草枯 丙酮酸乙酯 |
Protective effects of ethyl pyruvate treatment on lung injury in paraquat-intoxicated C57BL mice |
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| SHAO Yang,LUO Zhi-bin,GAO Jie,HE Jian-chuan,LIU Shi-ping,YOU Jian.Protective effects of ethyl pyruvate treatment on lung injury in paraquat-intoxicated C57BL mice[J].The Journal of Practical Medicine,2010,26(7). |
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| Authors: | SHAO Yang LUO Zhi-bin GAO Jie HE Jian-chuan LIU Shi-ping YOU Jian |
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| Institution: | SHAO Yang,LUO Zhi-bin,GAO Jie,HE Jian-chuan,LIU Shi-ping,YOU Jian.Affiliated Hospital of North Sichuan Medical College,Nanchong 637000,China |
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| Abstract: | Objective To investigate the mechanism of lung injury in paraquat-intoxicated C57BL mice,and the protective effects of ethyl pyruvate on lung injury.Methods Animals were randomly divided into 3 groups:control group(NS group),paraquat-intoxicated group(PI group),and PI followed by ethyl pyruvate(EP) treatment group(EP group).The C57BL mice in PI group received intraperitoneal injection of 20 mg/kg paraquat(PI group),and the C57BL mice in EP group treated with ethyl pyruvate(40 mg/kg,ip) 2 h after intraperito... |
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| Keywords: | Lung diseases Paraquat Ethyl pyruvate |
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