OX40基因rs17568A/G多态性与急性冠脉综合征的关联研究

目的研究OX40基因rs17568A/G多态性在中国汉族人群中的分布以及与急性冠脉综合征(acutecoronary syndrome,ACS)的相关性。方法共纳入228名中国汉族ACS患者和165名年龄性别匹配的正常对照,采用聚合酶链反应-限制性片断长度多态性(PCR-RFLP)技术对OX40基因rs17568A/G多态性位点进行基因型分型,同时采用DNA测序对酶切产物进行鉴定。结果ACS组和对照组中均检出了AA、AG和GG3种基因型,ACS组和对照组中rs17568A/G多态位点G等位基因频率分别为29.4%和26.7%,差异无统计学意义(P>0.05)。同时,基因型分布频率差异亦无统计学意义(P>0.05)。在ACS组中,根据冠脉病变分组,在1、2或3支血管病变患者间G等位基因和基因型频率也没有发现差异。通过不同基因型的血脂分析发现,G等位基因携带者(AG GG型)高密度脂蛋白胆固醇(high density cholesterol,HDL-C)水平显著高于AA纯合子(P<0.05)。结论OX40基因rs17568A/G多态性位点同中国汉族ACS的发生以及冠脉病变严重度间没有关联,但是G等位基因对于血清HDL-C水平有一定影响。

Correlation of OX40 Gene rs17568A/G Polymorphism with Acute Coronary Syndrome in Chinese Han Population

Objective To study the correlation of OX40 Gene rs17568A/G polymorphism with acute coronary syndrome in Chinese Han population.Methods 228 ACS patients and 165 matched controls of Chinese Han population were selected to study the OX40 gene rs17568A/G polymorphism with PCR-RFLP method.DNA sequences of enzyme digested products were also analyzed.Results All three genotypes,AA,AG and GG,were existed both in ACS patients and control group.OX40 gene rs17568A/G polymorphism frequencies were 29.4% for patients and 26.7% for control group respectively,in which the statistic significance was not observed(P>0.05).Besides,no significant differences were found for G allele and genotype frequencies in different severity of coronary lesion.But the results demonstrated that the presence of G allele was associated with high density lipoprotein(HDL)cholesterol concentration in either patients or controls.Conclusions OX40 gene rs17568A/G polymorphism was not associated with acute coronary syndrome in Chinese population,however,this polymorphism has some influence on serum HDL-C level in Chinese population.