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基因型和核苷酸序列分析研究丙型肝炎病毒母婴传播
引用本文:陆建春,陆志檬.基因型和核苷酸序列分析研究丙型肝炎病毒母婴传播[J].中华传染病杂志,1996,14(4):207-210.
作者姓名:陆建春  陆志檬
作者单位:上海第二医科大学瑞金医院,江苏省常州第三人民医院,江苏省常州市妇产医院
摘    要:为研究丙型肝炎病毒(HCV)的母婴传播,应用EIA方法检测1816例孕妇血清抗-HCV抗体。结果抗体阳性者16例(0.88%),进一步用逆转录-套式-聚合酶链反应(RT-Nested-PCR)检测病毒核酸(HCVRNA)14例孕妇为阳性,对其中13例孕妇所生13例婴儿随访至出生后6个月,3例婴儿检出HCVRNA,其中2例为短暂HCV感染者,1例为慢性HCV感染者伴ALT异常,母婴传播率为23.0%

关 键 词:丙型肝炎病毒  母婴传播  基因型  核苷酸

StudyonverticaltransmissionofhepatitisCviruswithanalysisofgenotypeandnucleotidese-quences
LuJianchun,LuZhimeng,QiuJihui,et al..StudyonverticaltransmissionofhepatitisCviruswithanalysisofgenotypeandnucleotidese-quences[J].Chinese Journal of Infectious Diseases,1996,14(4):207-210.
Authors:LuJianchun  LuZhimeng  QiuJihui  
Institution:LuJianchun,LuZhimeng,QiuJihui,etal.DepartmentofVirusResearch,RuijinHospi-tal,Shanghai200025
Abstract:ToresearchforverticaltransmissionofhepatitisCvirus,serumsamplesfrom1816pregnantwomenweretestedforanti-HCVantibodybyEIA.16(0.88%)werepositive.ThelatterweretestedforHCVRNAbyRT-Nested-PCR,and14werefoundHCVRNApositive.13babiesborntothe13HCVRNApositivepregnantwomenwerefolowedfor6months,3babieswereposi-tiveforHCVRNA,theverticalfransmissionratewas23.0%(3/13),amongthem2werefransientHCVinfection,and1waschronicHCVinfectionwithelevatedALT.HCVgenotypesweredetectedandthenucleotidesequencesoftheregionoftheHCVgenomespanningthecoreandenvelopegenesweredeterminedandcompared.TheresultsindicatethatverticaltransmissionofHCVisexistent.
Keywords:HepatitisCvirusVerticaltransmissionSequencing  
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