两种溃疡性结肠炎小鼠模型的构建及其炎症反应机制比较

  目的  构建葡聚糖硫酸钠(dextransulfatesodium, DSS)以及恶唑酮(oxazolone, OXZ)诱导的小鼠溃疡性结肠炎模型, 比较两种模型中炎症因子水平的变化, 评价两种模型。  方法  12只C57BL小鼠, 随机分为两组, DSS模型组(n=8)予3%DSS自由饮用5d, DSS对照组(n=4)饮用蒸馏水5d, 第6天处死两组小鼠。8只BALB/C小鼠, 随机分为两组, OXZ模型组(n=4)予皮肤涂抹3%OXZ(100%乙醇溶剂)0.2ml 2d, 5d后以0.8%OXZ(50%乙醇溶剂)0.15ml灌肠; OXZ对照组予皮肤涂抹100%乙醇0.2ml 2d, 5d后以50%乙醇0.15ml灌肠, 灌肠后第3天处死两组小鼠。观察各组小鼠疾病活动指数(disease activity index, DAI)、结肠组织大体评分和病理评分, 并检测小鼠结肠组织中髓过氧化物酶(myeloperoxidase, MPO)、白细胞介素-4(interleukin4, IL-4)、干扰素-γ(interferon-γ, IFN-γ)、肿瘤坏死因子-α(tumor necrosis factor-α, TNF-α)、核因子κB(nuclear factor-Kappa B, NF-κB)的水平。  结果  两种模型组小鼠DAI、组织大体评分和病理评分均较对照组有明显改变; DSS结肠炎、OXZ结肠炎均可导致MPO、TNF-α、NF-κB明显上升, DSS结肠炎IFN-γ明显上升, OXZ结肠炎IL-4明显上升。  结论  DSS及OXZ均能诱导出小鼠溃疡性结肠炎模型, 其中OXZ诱导的小鼠溃疡性结肠炎是Th2型炎症反应, 更接近人类溃疡性结肠炎。

Establishment of Two Murine Models of Ulcerative Colitis and Comparison of the Mechanism of Colitis-related Inflammatory Reactions

Objective To establish two murine models of ulcerative colitis (UC) and explore the mechanism of colitits-related inflammatory reactions. Methods Totally 12 C57BL mice were randomly divided into two groups:dextransulfatesodium (DSS) model group (n=8,allowed to drink 3% DSS for 5 days) and DSS control group (n=4,allowed to drink water for 5 days). In addition,8 BALB/C mice were randomly divided into oxazolone (OXZ) model group(n=4,mice were skin-sensitized with 3% OXZ 0.2 ml in 100% ethanol for 2 days follow...