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Comparative Evaluation of the Diagenode Multiplex PCR Assay on the BD Max System versus a Routine In-House Assay for Detection of Bordetella pertussis
Authors:Juliet Kenicer  Alison Hardie  Fiona Hamilton  Naomi Gadsby  Kate Templeton
Affiliation:Department of Medical Microbiology, Royal Infirmary of Edinburgh, Edinburgh, United Kingdom
Abstract:This study looked at 128 nasopharyngeal aspirates (NPA) and 162 throat swabs (TS) tested with the Diagenode multiplex assay on the BD Max system versus our in-house Bordetella pertussis PCR. Sensitivity and specificity were 97.3% and 100% for NPA and 88.3% and 98% for TS, respectively. Of positive NPA, 42.1% were coinfected with respiratory viruses.
Keywords:
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