Protective effects of bifidobacterial adhesin on intestinal mucosa of stressed male rats via modulation of inflammation |
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Authors: | Xiao-Liang Shu Tin-Tin Yu Kai Kang Han Xu Tao Lei |
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Affiliation: | 1.Department of Nutrition, East Hospital, Tong Ji University School of Medicine, Shanghai, 200000, China;2.Department of Endorine, Tong Ji Hospital, Tong Ji University School of Medicine, Shanghai, 200000, China |
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Abstract: | This study aimed to assess BA impact on inflammation markers and repair of intestinal mucosa. Forty-eight rats were randomly divided into stress (n = 24) and BA (n = 24) groups. Stress was induced by fettering in all animals, fed enterally with 125.4 kJ/kg/d and 0.2 g/kg/d nitrogen. Then, rats were treated for 8 days with 5 mg/kg/d BA (BA group) or 5 mg/kg/d saline (Stress group). Levels of NF-κB, IL-10, TNF-α, and IFN-γ were measured at different time points, in plasma and intestinal mucosa samples. Changes in intestinal mucosa morphology were observed by electron microscopy. Plasma and/or mucosal levels of NF-κB, TNF-α, and IFN-γ were significantly higher in both groups after stress induction (P < 0.05). These high levels persisted in control animals throughout the experiment, and were significantly reduced in the BA group, 3 and 8 days after stress induction (P < 0.05). Interestingly, IL-10 levels were increased after BA treatment (P < 0.05). At day 8, ileal mucosal villi and crypt structure were significantly restored in the BA group. Bifidobacterial adhesin plays a role in repairing intestinal mucosa injury after stress by regulating the release of inflammatory mediators in the intestinal mucosa. |
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Keywords: | Bifidobacterial adhesion, restrain stress, intestinal mucosa, NF-κ B, cytokines |
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