西安和郑州地区丙型肝炎患者的HCV基因分型

目的 了解西安和郑州地区丙型肝炎病毒（HCV）感染的基因型，比较了两地基因型分布的差异。方法 采用限制性长度多态性分析（RFLP）法对采自西安地区的46份和郑州地区的40份HCVRNA阳性标本进行HCV基因型分型研究。结果 西安地区46份标本中，25份（54．3％）为1b型病毒，17份（37．0％）为2a型病毒，4份（8．7％）为1b/2a混合型病毒；郑州地区40份标本中，19份（47．5％）为1b型，13份（32．5％）为2a型，3份（7．5％）为1a型，4份（10．0％）为1b/2a混合型病毒，1份（2．5％）为1a/1b混合型病毒。结论 两地的优势株均为HCV1b型，其次为2a型，郑州地区有1a型菌株的发现。

Genotyping hepatitis C virus isolates from Xi'an and Zhengzhou

AIM To investigate the genotypes of HCV in the HCV infected samples in Xi'an and Zhengzhou. METHODS Genotypes of HCV were determined by restriction fragment length polymorphism (RFLP) analysis of the 5' untranslated regions (UTR) of different HCVs. Forty six HCVRNA positive samples from Xi'an and forty from Zhengzhou were studied. RESULTS Among the forty six samples from Xi'an: 25/46 (54.3%) were found to be of 1 b genotype, 17/46 (37.0) were 2 a genotype and 4/46 (8.7%) were mixed genotype with 1 b and 2 a. Among those from Zhengzhou: 19/40 (47.5%) were 1 b, 13/40 (32.5%) were 2 a, 3/40 (7.5%) were 1 a, 4/40 (10%) were mixed genotype with 1 b and 2 a, 1/40 (2.5%) were mixed genotype with 1 a and 1 b. CONCLUSION In both Xi'an and Zhengzhou, the predominant isolates are genotype 1 b, followed by 2 a. Cases of genotype 1 a were found in Zhengzhou.