3种细胞条件培养基促鸡胚背根神经节生长的对照研究

探讨体外培养条件下，3种细胞条件培养基对鸡胚背根神经节生长的影响。方法：收集涎腺腺样囊性癌细胞系SACC-83、鼠成骨细胞系MC3T3-E1、人原代培养牙龈成纤维细胞HGF3种细胞24h条件培养基，对背根神经节进行培养，观察神经节神经元细胞及突起的生长及细胞迁出的情况。结果：培养36h观察发现，SACC-83有明显的促神经节突起生长作用；MC3T3-El不仅具有促突起生长作用，同时具有促DRG细胞迁出作用；HGF几无上述作用。结论：不同的细胞条件培养基对神经节的生长具有不同的作用，这种作用可能与不同细胞分泌的神经营养活性物质及其它营养因子的多少有关。

Studies on the effects of conditioned medium from three kinds of cell lines on the growth of DRG

Objective:To determine if three kinds of cell lines can affect the outgrowth of neuronal processes from dorsal root ganglia(DRG)in vitro.Method:Dorsal root ganglia were cultured by conditioned medium from SACC-83,MC3T3-E1 or HGF and the outgrowth of neuronal processes from DRG were observed.Result:After 36 hours' culture,SACC-83 had significantly promoted the outgrowth of neuronal processes in contrast to control groups.MC3T3-E1 not only could promote the outgrowth of neuronal processes,but also could facilitate the emigration of cells from DRG.HGF had neither effects.Conclusion:Different cell conditioned medium has different effects on the outgrowth of neuronal processes and the effects probably are relative to different neurotrophic factors they produce.