周围神经缺血后相关神经元凋亡及神经生长因子动态变化的研究

目的探讨大鼠坐骨神经缺血后相关脊髓节段和背根节神经元的凋亡及神经生长因子（NGF）表达的变化,以期为周围神经缺血性神经病的机制及临床治疗提供理论依据。方法结扎大鼠单侧髂总动脉制作坐骨神经缺血模型,分别于术后3、5、7、14、21、28d用4%多聚甲醛灌流固定,取腰髓L4～6节段和双侧第4～6腰脊神经节固定,分别进行免疫组织化学及TUNEL染色检测神经元的NGF和凋亡表达,同体对照。结果坐骨神经缺血的不同时间段L4～6脊髓前角运动神经元及背根节神经元可见凋亡神经元细胞,背根节神经元凋亡率术后3d时显著性升高,术后7d时达到峰值后缓慢下降,后期接近对照侧水平。腰髓前角运动神经元在术后5d时开始有显著性提高,14d时达到最大值后逐渐下降,后期较对照侧仍显著性增高。腰髓前角运动神经元及背根节神经元NGF的表达5d时阳性率较对照侧开始显著降低,7d时达到最低点后逐渐复原,至术后28d时与对照侧无明显差异。结论周围神经缺血性损伤导致相关神经元胞体中NGF含量减少,与神经元凋亡相关。缺血性损伤对背根节感觉神经元的影响较脊髓运动神经明显。

Study on the apoptosis of related neuron and the changes of nerve growth factor after peripheral nerve ischemia in rats

Objective To observe the apoptosis of related neuron and the changes of expression of nerve growth factor(NGF) in lumbar segment and dorsal root ganglion(DRG) neurons after peripheral nerve ischemia in rats,and to investigate the relationship between neuron apoptosis and neurotrophic factors(NTFs) in order to provide theoretical basis for the study on peripheral neuropathy in basic medicine and clinical medicine.Methods The animal models of peripheral nerve ischemia in Wistar rats were made by ligating expe...