Lectin histochemistry for the study of glycoconjugate residues on colonic adenocarcinoma |
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| Affiliation: | (1) Department of Pathology, National Institute of Health, 1-23-1, Toyama, Shinjuku-ku, 162 Tokyo, Japan;(2) Department of Cell and Molecular Pathology, Institute of Pathology, University of Zürich Medical School, Schmelzbergstr. 12, CH-8091 Zürich, Switzerland |
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| Abstract: | TheMaackia amurensis leukoagglutinin (MAL),Sambucus nigra L.I (SNA I), andAmaranthus caudatus (amaranthin; ACL) lectins were used for the study of glycoconjugate residues on normal colonic mucosa and adenocarcinoma by the lectin gold technique. MAL labeled normal colonic mucosa but SNA I did not. ACL stained normal mucosa with regional differences. In contrast, all three lectins labeled most adenocarcinomas. The labeling pattern of MAL was variable but that of SNA I and ACL bound the apical surface of carcinoma cells. Some cells were labeled by SNA I but not by MAL, suggesting thatde novo expression of an α2, 6 sialytrans-ferase was accompanied by malignant transformation. Pretreatment of the sections with galactose oxidase-Schiff sequence abolished the ACL labeling, indicating colonic mucosa and carcinoma cells exhibiting a cryptic form of the T antigen. |
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| Keywords: | Lectin histochemistry Colon cancer-Sambucus nigra L. I Maackia amurensis L. Amaranthus caudatus L. |
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