抗HIV-1 gp120单克隆抗体体外阻断CD4细胞HIV感染的研究

目的:抗HIV-1gp120单克隆抗体对细胞感染HIV的拮抗作用研究。方法:制备抗HIV-1gp120单克隆抗体,鉴定其特性;研究抗HIV-1gp120单克隆抗体对细胞感染HIV的拮抗作用。结果:构建的重组质粒的外源基因片段经测序与预期HIV-1gp120抗原基因片段大小一致;高浓度时该单克隆抗体能够抑制HIV病毒的感染,随着抗体浓度的降低,中和实验的抑制率也随之下降;高浓度时该单克隆抗体能够显著抑制病毒的感染,随着抗体浓度的降低,HIVgp120RNA定量增高。结论:获得6株稳定分泌抗HIV-1gp120单克隆抗体的杂交瘤细胞株,该抗HIV-1gp120单克隆抗体具有一定的抗HIV作用。

Study of HIV-1 gp120 monoclonal antibody blocking HIV infection of CD4 lymphocyte in vitro experiment

Objective：To investigate HIV-1 gp120 monoclonal antibodies’ antagonistic effect on HIV infection.Method：Prepare HIV-1 gp120 monoclonal antibody and identify it.Investigate HIV-1 gp120 monoclonal antibodies’ antagonistic effect on HIV infection.Results：Exogenous gene in recombinant plasmid is in consistent with expected gene in HIV-1 gp120 antigen detected by sequencing.Monoclonal antibody in higher concentration may inhibite virus infection significantly,and as monoclonal antibody concentration is decreasing,results of quantitative detection of HIV gp120 RNA is increasing.Conclusion：6 cell strains of hybridoma secreting monoclonal antibody consistently are obtained,and which have certain activities against HIV infection.