抗HIV-1 gp120单克隆抗体体外阻断CD4细胞HIV感染的研究 |
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引用本文: | 赵磊,赵伟,张永成,张亮,文剑. 抗HIV-1 gp120单克隆抗体体外阻断CD4细胞HIV感染的研究[J]. 陕西医学杂志, 2010, 39(7): 797-801 |
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作者姓名: | 赵磊 赵伟 张永成 张亮 文剑 |
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作者单位: | 东南大学第二附属医院,南京,210003 |
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摘 要: | 目的:抗HIV-1gp120单克隆抗体对细胞感染HIV的拮抗作用研究。方法:制备抗HIV-1gp120单克隆抗体,鉴定其特性;研究抗HIV-1gp120单克隆抗体对细胞感染HIV的拮抗作用。结果:构建的重组质粒的外源基因片段经测序与预期HIV-1gp120抗原基因片段大小一致;高浓度时该单克隆抗体能够抑制HIV病毒的感染,随着抗体浓度的降低,中和实验的抑制率也随之下降;高浓度时该单克隆抗体能够显著抑制病毒的感染,随着抗体浓度的降低,HIVgp120RNA定量增高。结论:获得6株稳定分泌抗HIV-1gp120单克隆抗体的杂交瘤细胞株,该抗HIV-1gp120单克隆抗体具有一定的抗HIV作用。
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关 键 词: | HIV-1/免疫学 HIV包膜蛋白质gp120/免疫学 抗体,单克隆 聚合酶链反应 @细胞,CD4 |
Study of HIV-1 gp120 monoclonal antibody blocking HIV infection of CD4 lymphocyte in vitro experiment |
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Affiliation: | Clinical Laboratory Department,Second Affiliated Hospital of Southeastern University(Nanjing 210003) Zhao Lei Zhao Wei Zhang Yongcheng et al |
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Abstract: | Objective:To investigate HIV-1 gp120 monoclonal antibodies’ antagonistic effect on HIV infection.Method:Prepare HIV-1 gp120 monoclonal antibody and identify it.Investigate HIV-1 gp120 monoclonal antibodies’ antagonistic effect on HIV infection.Results:Exogenous gene in recombinant plasmid is in consistent with expected gene in HIV-1 gp120 antigen detected by sequencing.Monoclonal antibody in higher concentration may inhibite virus infection significantly,and as monoclonal antibody concentration is decreasing,results of quantitative detection of HIV gp120 RNA is increasing.Conclusion:6 cell strains of hybridoma secreting monoclonal antibody consistently are obtained,and which have certain activities against HIV infection. |
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Keywords: | HIV-1/immunology HIV envelope protein gp120/immunology Antibodies monoclonal Polymerase chain reation @Cell CD4 |
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