胶质瘤组织中多药耐药性相关蛋白的表达变化及意义

目的观察胶质瘤组织中耐药蛋白谷胱甘肽S转移酶π（GST-π）、O6-甲基鸟嘌呤DNA甲基转移酶（MGMT）在胶质瘤组织中的表达变化,为临床制订个体化化疗方案提供依据。方法选取69例胶质瘤病理切片,采用免疫组化SP法检测GST-π、MGMT,分析其与胶质瘤病理级别的关系及两者表达的相关性。结果 69例胶质瘤组织中GST-π阳性表达率为72.46%（50/69）,其表达强度与病理分级呈正相关（r=0.374,P〈0.05）;MGMT阳性率为63.77%（44/69）,其表达强度与胶质瘤病理分级无关（P〉0.05）。GST-π与MGMT的表达无相关性（P〉0.05）。结论 GST-π的表达与胶质瘤恶性程度有关,MGMT与恶性程度无关。检测胶质瘤组织中GST-π、MGMT有利于指导临床进行个体化化疗。

Expressions and significance of correlative proteins on glioma multidrug resistance

Objective To study the expression of glutathione S-transferase л（GST-π） and O6-methylguanine-DNA methyltransferase（MGMT） in glioma tissue and to provide experimental data for making individua-lized clinical chemotherapy regimens.Method The expression of GST-π and MGMT in 69 cases glioma tissue were detected by immunohistochemical SP method.Results The positive expression rate of GST-π was 72.46%（50/69）,expression intensity was positively correlated with pathological grade（r=0.374,P〈0.05）.The positive expression rate of MGMT was 63.77%（44/69）,expression intensity had nothing to do with pathological grades（P〉0.05）.There was no correlation between the expression of GST-π and MGMT in glioma（P〉0.05）.Conclusions The expression of GST-π in glioma is related with its malignance,but MGMT is not;It is preferable to instruct individualized clinical chemotherapy by assaying simultaneously the expression of GST-π and MGMT.