| 高效液相色谱法测定妇科洗剂中盐酸小檗碱的含量 |
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| 引用本文： | 杨柳,李婷,张义生,孙晓静,李力,王薇,解小霞. 高效液相色谱法测定妇科洗剂中盐酸小檗碱的含量[J]. 湖北中医药大学学报, 2017, 19(4). DOI: 10.3969/j.issn.1008-987x.2017.04.08 |
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| 作者姓名： | 杨柳 李婷 张义生 孙晓静 李力 王薇 解小霞 |
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| 作者单位： | 武汉市中医医院/国家中医药管理局中药制剂三级实验室,湖北武汉,430014 |
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| 基金项目： | 2016年度武汉市临床医学科研项目，武汉市中青年医学骨干人才培养工程 |
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| 摘 要： | 目的建立妇科洗剂中盐酸小檗碱的含量测定方法。方法应用高效液相色谱(HPLC)法,采用Wondasil C_(18)色谱柱(250mm×4.6mm,5μm);以乙腈-0.1磷酸(50:50)(每100mL加十二烷基磺酸钠0.2g)为流动相,检测波长为265nm;柱温30℃。结果盐酸小檗碱进样量在132.40-662.00ng范围内与峰面积有良好的线性关系(r=0.9999),平均加样回收率为100.97%,RSD值为2.76%(n=9)。结论该方法快速、准确,操作简单,专属性强,可作为妇科洗剂中盐酸小檗碱的含量测定方法。
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| 关 键 词： | 妇科洗剂 盐酸小檗碱 高效液相色谱 |
Determination of Herberine Hydrochloride in Gynecological Lotion by HPLC |
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| YANG Liu,LI Ting,ZHANG Yisheng,SUN Xiaojing,LI Li,WANG Wei,XIE Xiaoxia. Determination of Herberine Hydrochloride in Gynecological Lotion by HPLC[J]. Journal of Hubei University of Chinese Medicine, 2017, 19(4). DOI: 10.3969/j.issn.1008-987x.2017.04.08 |
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| Authors: | YANG Liu LI Ting ZHANG Yisheng SUN Xiaojing LI Li WANG Wei XIE Xiaoxia |
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| Abstract: | Objective To establish a determination method ofberberine hydrochloride in Gynecological lotion by HPLC.Methods HPLC was used with Wondasil C18(250mm× 4.6 mm,5μm) as the analytical column.The mobile phase was composed of acetonitrile-0.1％ phosphric acid solution(50:50)(0.2g sodium dodecylsulphate was added into the 100 mL mobile phase).The detection wavelength was set at 265nm and the column temperature was 30℃.Results The calibration curve ofberberine hydrochloride was in good linearity over the ranges of 132.40～662.00ng (r=0.9999).The average recovery rate was 100.97％ (RSD=2.76％).Conclusion The method is simple,feasible and reproducible,which is suitable for the quality control of Gynecological lotion. |
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| Keywords: | Gynecological lotion berberine hydrochloride HPLC |
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