UPLC法同时测定淫羊藿中5种黄酮类成分的含量

目的建立同步测定中药材淫羊藿中朝藿定A、朝藿定B、朝藿定C、淫羊藿苷、宝藿苷I 5种黄酮类成分含量的UPLC-DAD法。方法采用Aglientl290型超高效液相色谱仪,色谱柱为Aglient Zorlbax EclipsePlus C_(18)柱(50mm×2.1mm,1.8μm),柱温为40℃。流动相为乙腈和0.1%磷酸水,梯度洗脱,流速为0.4mL/min,进样量为1μL,检测波长为270nm。结果淫羊藿药材中5种黄酮类成分分别在2.02~202μg/mL、2.12~212μg/mL、2.14~214μg/mL、2.52~252μg/mL和1.12~112μg/mL的浓度范围内呈良好的线性关系,最低检测限分别为2.82、3.03、2.72、3.09、1.23ng/mL。重复性、稳定性试验结果的RSD值均小于3%,各成分平均回收率分别为99.76%、98.96%、98.76%、98.67%和98.65%。同时对14批不同药房或产地的药材中5种黄酮类成分的含量进行了检测。结论该方法快速简便、准确可靠,各主要化学成分均能达到基线分离,适用于淫羊藿药材的多成分分析,进而为淫羊藿药材质量控制的全面评价提供科学依据。

Simultaneous Determination of Five Flavonoids in Epimedii Folium by UPLC

Objective To establish a UPLC-DAD method for simultaneous determination of the content of 5 flavonoids (epimedins A,B,C,icariin and baohuoside Ⅰ) in Epimedii Folium.Methods Aglient 1290 ultra performance liquid chromatography was used,the column was Aglient Zorbax Eclipse Plus C18 (50mm× 2.lmm,1.8μm),column temperature was 40℃.Mobile phase composed of acetonitrile and 0.1％ phosphate water with gradient elution at flow rate of 0.4mL/min,injection volumn was lμL,detection wavelength was 270nm.Results The linear ranges of 5 flavonoids were 2.02～202μg/mL,2.12～212μg/mL,2.14～214μJg/ml,2.52～252 μg/mL and 1.12～112μg/mL respectively with good linear relationships.The lowest detection limits were 2.82,3.03,2.72,3.09,1.23ng/mL respectively.The RSD results of repeatability and stability were less than 3％.The average recovery rates were 99.76％,98.96％,98.76％,98.67％ and 98.65％ respectively.The contents of 5 flavonoids from 14 different pharmacies of regions were also detected.Conclusion The method is rapid,simple,accurate and reliable,which can be used for quality control standard of Epimedii Folium.