甘草地上部分水提液总黄酮纯化工艺研究

目的研究大孔树脂纯化甘草地上部分水提液中总黄酮的工艺。方法通过对D-101、AB-8、DM-130、HPD-BJQH、HPD-600、HPD-100和HPD-450等7种树脂对总黄酮的静态吸附与解吸附考察,优选适宜类型的大孔树脂,并进一步对富集纯化参数进行考察及优化。结果选用AB-8型大孔树脂,优选的工艺条件为:上样液质量浓度2.128 mg/m L,样品液p H5.42,树脂的上样量为7 m L/g,上样速度为1.5 BV,依次用2 BV水洗脱,4 BV 60%乙醇洗脱,经AB-8树脂处理后的甘草地上部分水提液的总黄酮纯度由9.08%提升到34.02%,收率为74.40%。结论 AB-8型大孔树脂能有效地纯化富集甘草地上部分水提液总黄酮,且此工艺稳定。

Study on Purification Process of Total Flavonoids of Water Extract from the Aerial Parts of Licorice

Objective To investigate the purification technology of total flavonoids of water extract from the aerial parts of licorice.Methods Seven macroporous resins (D-101、AB-8、DM-130、HPD-600、HPD-100 and HPD-450) were chosen with static and dynamic adsorption and desorption experiments to optimize the purification parameters.Result AB-8 macroporous resin was chosen and its optimized technology conditions were as followings:the concentration of sample solution 2.128 mg/mL(pH=5.42),the loaded amount of 7mL/g,and loading flow rate of 1.5 BV/h.The sample was eluted by distilled water with 2 BV and 60％ ethanol of 4BV,respectively.The purity of total flavonoids was increased from 9.08％ to 34.02％ after the purification,and the yield was 74.40％.Conclusion AB-8 is an ideal resin with the best enrichment for separating and purifying the total flavonoids of water extract from the aerial parts of licorice.