头针对心肌缺血再灌注损伤大鼠心肌酶谱的影响

目的观察头针"额旁1线"对心肌缺血再灌注损伤大鼠血浆乳酸脱氢酶(LDH)、心肌Na~+-K~+-ATPase、Ca~(2+)-Mg~(2+)-ATPase活性的影响,探讨头针防治心肌缺血再灌注损伤的作用机制。方法Wistar大鼠随机分为空白对照组、假手术组、缺血再灌组和头针治疗组,每组8只。采用结扎左冠状动脉前降支30min,再灌注15min制备大鼠心肌缺血再灌注模型。取头部双侧"额旁1线"区域进行电针治疗,频率14Hz,强度2V。以血浆乳酸脱氢酶(LDH)、心肌Na~+-K~+-ATPase和Ca~(2+)-Mg~(2+)-ATPase活性为观察指标。结果模型组大鼠较假手术组、空白对照组血浆LDH活性明显升高(P0.01),而头针治疗组较模型组明显降低(P0.01)。模型组心肌Na~+-K~+-ATPase、Ca~(2+)-Mg~(2+)-ATPase活性显著下降,与假手术组、空白对照组比较差异有统计学意义(P0.01),而头针治疗组与模型组比较,这两种酶活性显著提高(P0.01)。结论头针具有防治心肌缺血再灌性损伤的作用,其机制可能与保护各种心肌酶类,减轻Ca~(2+)超载有关。

Effects of Scalp Acupucture on Mycardial Enzymes in Mycardial Ischemia-Reperfusion Injury Rats

Objective To explore the mechanism of scalp acupucture (SA) in the prevention of myocardial ischemia-reperfusion injury (MI/RI)by examing the activity of plasma lactate dehydrogenase(LDH),myocardial Na +-K + -ATPase and Ca2 +-Mg2 +-ATPase.Methods 32 rats were randomly divided into the blank control,sham,model and SA treatment groups (n =8).MI/RI model was induced by ligation of the left anterior descending coronary artery for 30min,followed by release of the ligation for 15min.SA was applied to bilateral Epangxian 1 for 30 min.The activity of LDH,myocardial Na +-K +-ATPase and Ca2 +-Mg2 +-ATPase were observed.Results Compared with blank control and sham group,the activity of LDH was significantly increased,whereas the activity of myocardial Na +-K +-ATPase and Ca2+-Mg2+-ATPase were markedly decreased in model group.In comparison with model group,the activity of LDH was obviously lowered,whereas the activity of myocardial Na +-K +-ATPase and Ca2+-Mg2+-ATPase were clearly enhanced in scalp treatment group.Conclusion Scalp acupucture can prevent myocardial ischemia-reperfusion injury,and its mechanism may be related to the protection of various enzymes class,thus reducing the Ca2 + overload.