风寒湿对类风湿关节炎DNA甲基化和组蛋白修饰的影响

目的探讨类风湿关节炎(RA)风寒湿病证结合模型的造模方法及风寒湿因素对RA DNA甲基化和组蛋白修饰的影响。方法在胶原诱导性关节炎(CIA)和风寒湿因素刺激的基础上建立RA风寒湿病证结合模型,进行关节评分、测关节直径、检测外周血单个核细胞(PBMCs)DNA甲基化及组蛋白H3乙酰化水平。结果与正常组相比,风寒湿病证结合模型组关节炎指数评分≥5分的比例显著升高(P0.05);大鼠踝关节直径显著增加(P0.05)。与CIA模型组相比,风寒湿病证结合模型组PBMCs DNA甲基化水平显著下降(P0.05);PBMCs组蛋白H3乙酰化水平有所升高,但无统计学意义。结论风寒湿因素可能通过下调PBMCs DNA甲基化水平,从而影响关节炎严重程度。

Effects of Wind-cold-dampness on DNA Methylation and Histone Modifications in Rats with Collagen-induced Arthritis

Objective To establish a wind-cold-dampness (WCD) model of rheumatoid arthritis (RA) and explore the effects of WCD on DNA methylation and histone modifications in rats with collagen-induced arthritis (CIA).Methods The WCD syndrome of RA was established based on CIA and WCD stimulation.Meanwhile, arthritis index (AI), assay ankle diameter, detect global DNA methylation and histone H3 acetylation in peripheral blood mononuclear cells (PBMCs) were evaluated.Results The proportion of AI score ≥5 in group WCD was significantly higher than that in normal group (P< 0.05).The ankle diameter was significantly increased in group WCD than that in normal group (P< 0.05).Compared with group CIA, the DNA methylation level was significantly reduced in group WCD (P< 0.05).Compared with group CIA, the total histone H3 acetylation level was enhanced in group WCD;however, there was no significant difference between the two groups.Conclusion WCD influences the severity of RA may through reducing the level of DNA methylation in PBMCs.