| H_2O_2对SD大鼠海马AMPA受体棕榈酰化水平的影响 |
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| 引用本文： | 韩俊,张英,黄妍,但汉雄. H_2O_2对SD大鼠海马AMPA受体棕榈酰化水平的影响[J]. 湖北中医药大学学报, 2017, 19(5). DOI: 10.3969/j.issn.1008-987x.2017.05.02 |
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| 作者姓名： | 韩俊 张英 黄妍 但汉雄 |
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| 作者单位： | 1. 江汉大学附属医院药剂科,湖北武汉,430015;2. 湖北中医药大学药学院,湖北武汉,430065 |
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| 摘 要： | 目的研究H_2O_2对SD大鼠海马-氨基-3-羟基-5-甲基-4-异恶唑丙酸(AMPA)受体棕榈酰化水平的影响。方法用400 M H_2O_2孵育正常大鼠急性脑片后,分离海马组织;酰基生物素交换技术提取AMPA受体亚基GluA1、GluA2棕榈酰化蛋白;蛋白免疫印迹技术检测上述蛋白表达水平。结果与对照组相比,H_2O_2升高大鼠海马蛋白棕榈酰化总水平、Glu A1(P0.05)和Glu A2(P0.01)棕榈酰化水平。结论400 MH_2O_2可显著提高SD大鼠海马AMPA受体棕榈酰化水平。
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| 关 键 词： | 海马 α-氨基-3-羟基-5-甲基-4-异(噁)唑丙酸受体 棕榈酰化 过氧化氢 |
The Effects of Palmitoylation of AMPA Receptors in Hippocampus of Rats by Hydrogen Peroxide |
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| HAN Jun,ZHANG Ying,HUANG Yan,DAN Hanxiong. The Effects of Palmitoylation of AMPA Receptors in Hippocampus of Rats by Hydrogen Peroxide[J]. Journal of Hubei University of Chinese Medicine, 2017, 19(5). DOI: 10.3969/j.issn.1008-987x.2017.05.02 |
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| Authors: | HAN Jun ZHANG Ying HUANG Yan DAN Hanxiong |
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| Abstract: | Objective To investigate the effects on palmitoylated modification of AMPA receptors in hippocampus of rats by hydrogen peroxide (H2O2).Methods The palmitoylated protein of GluA1,GluA2 in the hippocampus of the acute slices for rats after incubated 400μM H2O2 were extracted through acyl-biotinyl exchange method.The expression levels of extracted protein were detected by western blotting.Results Compared with the control rats,the global palmitoylated level in the hippocampus of the incubated H2O2 rats was significantly increased,as well as the palmitoylation of GluA1 (P＜0.05) and GluA2 (P＜0.0 1) in hippocampus of the incubated H2O2 rats were remarkably elevated.Conclusion The present study indicated that the palmitoylated levels of AMPA receptors in hippocampus of rats were increased by 400μ M H2O2. |
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| Keywords: | hippocampus AMPA receptors palmitoylation hydrogen peroxide |
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