活性煅烧骨颗粒骨水泥复合材料的弹性模量测定及其临床意义

背景：不同比例的复合材料其弹性模量不同，植入体内可由此产生应力遮挡，从而影响材料与宿主骨的生物结合，最终影响骨缺损的修复效果。 目的：对活性煅烧骨颗粒和骨水泥复合制成的组织工程骨进行弹性模量测定，为临床上修复不同部位的骨缺损提供实验依据。 设计：对照实验。 单位：解放军第四军医大学唐都医院全军骨肿瘤研究所。 材料：实验于2002—08—21／2003—03-12在解放军第四军医大学唐都医院全军骨肿瘤研究所完成：昆明种小鼠10只，体质量10～15g，雌雄不拘；新鲜1岁小牛长管状皮质骨；新鲜尸体股骨下端松质骨标本和新鲜尸体股骨中段标本各6个。 方法：提取牛骨形成蛋白并进行成骨诱导活性测定，制备有活性的煅烧骨颗粒，将牛骨形成蛋白与煅烧骨颗粒以1：25的质量比复合后，再与骨水泥按0：10，4：6，5：5，6：4和7．5：2．5的质量比进行复合，对活性煅烧骨颗粒和骨水泥复合制成的组织工程骨进行弹性模量测定，并与正常人成年男性的股骨．股骨下端松质骨的弹性模量相比较。 主要观察指标：弹性模量测定及比较结果。 结果：含活性煅烧骨颗粒60％的复合材料（活性煅烧骨颗粒：骨水泥为6：4）的弹性模量与正常成年男性股骨下端松质骨的弹性模量差异无显著性意义（P〉0.05），其余两两比较均差异有显著性意义成人股骨（6．2167±0．2229）MPa；松质骨（1．3517±0．3069）MPa；活性煅烧骨颗粒：骨水泥为0：10（5．7100±0．1663）MPa；活性煅烧骨颗粒：骨水泥为4：6（3.5101±0.2050）MPa；活性煅烧骨颗粒：骨水泥为5：5（20041±0．1500）MPa;活性煅烧骨颗粒：骨水泥为6：4（1．5018±0．0057）MPa；活性煅烧骨颗粒：骨水泥为7．5：2．5（0．9004±0．0251）MPa，P〈0．01]。 结论：含活性煅烧骨颗粒和骨水泥复合制成的组织

Measurement and clinical significance of elastic modulus of composite of true bone ceramic granule and bone cement

BACKGROUND： Composite with different ratios has various elastic moduli, and this can cause stress protection in implanted body so as to influence on biological integration between composite and host bone and plerosis of bone defection.
OBJECTIVE： To measure elastic modulus of tissue engineering bone made of composite between true bone ceramic （TBC） granule and bone cement （BC） and provide experimental data for repairing bone defection at various sites.
DESIGN： Controlled study.
SETTING： Military Institute of Bone Tumor, Tangdu Hospital of the Fourth Military Medical University of Chinese PLA.
MATERIALS： The experiment was carried out in the Military Institute of Bone Tumor, Tangdu Hospital of the Fourth Military Medical University of Chinese PLA from August 2002 to March 2003. Ten Kunming mice, weighing 10-15 g, of both genders, were selected in this study. Fresh longtubular cortical bone of 1-year calf, 6 samples of spongy bone at inferior extremity and 6 samples at middle femur of fresh corpse were also selected in this study.
METHODS： Bovine bone morphogenctic proteiu （hBMP） was extracted to measure osteogenic-induced activity and make TBC granule, bBMP was mixed with TBC as the ratio of 1：25, and then with BC as the ratios of 0： 10, 4：6, 5：5, 6：4 and 7.5：2.5. In addition, elastic modulus of tissue engineering bone made of TBC-BC composite was measured and compared with elastic modulus of femur and spongy bone of normal adult males.
MAIN OUTCOME MEASURES： Measurement and comparison of elastic modulus.
RESULTS： Elastic modulus of composite containing 60% TBC （Ratio between TBC and BC was 6：4） was no significantly different from that of spongy bone at inferior extremity of femur of normal adult males （P 〉 0.05）, but other comparisons of every two subjects were significant difference femur of the adults： （6.216 7±0.222 9） MPa; spongy bone： （1.351 7±0.306 9） MPa; TBC/BC （0：10）： （5.710 0±0.166 3） MPa; TBC/BC （4：6）：