| 实时荧光定量RT-PCR法测定脑胶质瘤中MT1-MMP基因mRNA表达水平 |
| |
| 引用本文： | 刘维生,耿会娟,王硕,肖新如,陈善文,赵继宗.实时荧光定量RT-PCR法测定脑胶质瘤中MT1-MMP基因mRNA表达水平[J].中国微侵袭神经外科杂志,2008,13(2):75-77. |
| |
| 作者姓名： | 刘维生 耿会娟 王硕 肖新如 陈善文 赵继宗 |
| |
| 作者单位： | 1. 首都医科大学附属北京天坛医院神经外科,北京,100050
2. 首都医科大学附属北京天坛医院检验科,北京,100050 |
| |
| 摘 要： | 目的探讨胶质瘤恶性程度和MT1-MMP基因mRNA表达水平的关系。方法采用实时荧光定量RT-PCR法检测34例脑胶质瘤和7例正常脑组织（取自癫痫病人手术切除脑组织）中MT1-MMP的mRNA表达水平。结果经实时荧光定量RT-PCR法检测，正常脑组织和低级别、高级别胶质瘤的△△Ct（循环阈值）值分别为（0．834±0、517）、（4．063±2．309）和（7.072±4．072）。正常脑组织与低级别、高级别胶质瘤之间，低级别、高级别胶质瘤之间MT1-MMP基因mRNA表达水平的差异具有统计学意义（P〈0．05）。结论脑胶质瘤中存在MT1-MMP基因mRNA高水平的表达，随着肿瘤恶性级别的增加，MT1-MMP基因mRNA表达水平有增高趋势。
|
| 关 键 词： | 神经胶质瘤 间质胶原酶 逆转录聚合酶链式反应 |
| 文章编号： | 1009-122X(2008)02-0075-03 |
| 修稿时间： | 2007年6月8日 |
mRNA expression of MT1-MMP gene in glioma by quantitative real-time fluorescence RT-PCR |
| |
| LIU Weisheng,GENG Huijuan,WANG Shuo,et al..mRNA expression of MT1-MMP gene in glioma by quantitative real-time fluorescence RT-PCR[J].Chinese Journal of Minimally Invasive Neurosurgery,2008,13(2):75-77. |
| |
| Authors: | LIU Weisheng GENG Huijuan WANG Shuo |
| |
| Institution: | LIU Weisheng1,GENG Huijuan2,WANG Shuo1,et al.1.Department of Neurosurgery,2.Department of Clinical Laboratory,Beijing Tiantan Hospital,Capital University of Medical Sciences,Beijing 100050,China |
| |
| Abstract: | Objective To explore the relationship between malignancy of glioma and mRNA expression of MT1-MMP gene. Methods The mRNA expression of MT1-MMP gene in 34 gliomas and 7 normal brain tissues （from epileptic focus resection） was detected by quantitative real-time RT-PCR method. Results By Real-time RT-PCR method, the △△Ct（cycle threshold） values of the normal brain tissue and low-grade, high-grade glioma were 0.834±0.517, 4.063±2.309, and 7.072±4,072 respectively. There was statistical significance of difference between normal brain tissue and low-grade, high-grade glioma, and between low-grade and high-grade glioma （P〈0.05）. Conclusion The mRNA expression of MT1-MMP gene is up-regulated in glioma, and increases with the malignancy grade of glioma. |
| |
| Keywords: | |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
