蕈样肉芽肿皮损中IL-10与树突状细胞关系的研究

目的探讨蕈样肉芽肿(MF)疾病进程中IL-10的变化特征与树突状细胞(DC)的关系。方法取皮肤MF肿瘤组织冰冻切片,采用单克隆抗体免疫组化染色(AEC,DAB,VIP单染,DAB/VIP双染及DAB/VIP/H istoG reen多染)及TdT介导的dUTP缺口末端标记技术(TUNEL)进行原位检测。结果MF肿瘤组织的斑片及斑块期真表皮内IL-10+细胞数明显低于肿瘤期(P<0.05),肿瘤期真皮浸润性T细胞区域可见许多IL-10+细胞,为两种不同的细胞。双染显示IL-10+细胞常聚集在未成熟的DC周围,多染发现吞噬有凋亡细胞的未成熟DC周围有IL-10+细胞存在。结论MF皮损中IL-10增多是这一疾病进展的重要征象,它可能至少有两种细胞分泌,在肿瘤期,IL-10可能通过阻止吞噬有凋亡细胞的DC的成熟诱导抗肿瘤免疫耐受。

The Relation Between IL-10 and Dendritic Cells in the skin Infiltrates of MF Patients

Objective To identify the changing situation of IL-10 in the skin infiltrates of Mycosis fungoides(MF) patients and the relation between IL-10 and dendritic cells(DC).Methods Mab and immunohistochemistry were used to detect the expression of special antigen and TUNEL for apoptosis in situ.Results The numbers of IL-10~+ cells in tumor stage(TS) were differently more than those in patch/plaque-stage MF(PS),(P<0.05).A equal numbers of IL-10~+cells were densely interspersed in the infiltrating dermis of tumor stage MF(TS) and those cells had two different morphology.Though two and three kinds of colors,we showed that IL-10~+ cells located near the immature DCs swallowing apoptotic cells.Conclusion The increase of IL-10~+ cells is an important sign in the progressive course of MF and it is secreted by two kinds of cells.To inhibit the mature of immature DCs that swallowed apoptotic tumor cells,it may be induce an anti-tumor immune tolerance.