丹参酮ⅡA联合奥沙利铂对人肝癌细胞SMMC-7721作用的实验研究

 目的探讨丹参酮ⅡA对人肝癌细胞系SMMC-7721生长活性及对奥沙利铂敏感性的影响和机制。方法不同浓度的丹参酮ⅡA和奥沙利铂单独或联合作用人肝癌细胞系SMMC-7721 72 h后,采用噻唑蓝(MTT)法检测该细胞系的生长活性,分析丹参酮ⅡA与奥沙利铂在抑制肝癌细胞增殖中的相互作用。流式细胞术分析丹参酮ⅡA单独及联合应用奥沙利铂对肝癌细胞凋亡的影响。结果实验所选各种浓度丹参酮ⅡA对SMMC-7721均表现出一定程度的生长抑制作用,并呈剂量依赖性;当丹参酮ⅡA质量浓度为1.0,2.0,5.0,10.0 mg·L^-1时,合用质量浓度为1.0,2.0,5.0 mg·L^-1的奥沙利铂能显著抑制SMMC-7721细胞的增殖,并显示出两种药物的协同作用(q>1.15)。流式细胞术分析发现两种药物均可有效诱导SMMC-7721细胞的凋亡,且在上述浓度联合应用时具协同效应。结论丹参酮ⅡA可以显著抑制肝癌细胞系SMMC7721细胞增殖,并能增强该细胞系对奥沙利铂的敏感性,其作用机制可能与丹参酮ⅡA和奥沙利铂协同诱导细胞凋亡有关。

Effect of Tanshinone Ⅱ A Combined with Oxaliplatin on Human Hepatocellular Carcinoma Cell Line SMMC-7721

OBJECTIVE To investigate the effects of tanshinone ⅡA on the growth activities and oxaliplatin sensitivity in human hepatocellular carcinoma cell line SMMC-7721 and its mechanism.METHODS After treating SMMC-7721 cells with different concentrations of tanshinone ⅡA with or without oxaliplatin for 72 h,MTT assay was used to examine the growth activities on these cells.The cooperative effects of tanshinone ⅡA and Oxaliplatin on inhibiting the growth of SMMC-7721 cells were analyzed.Flow cytometry(FCM) was used to examine the efgects of Tanshinone ⅡA alone and Tanshione combinated with oxaliplatin on the apoptosis of SMMC-7721 cells.RESULTS Tanshinone ⅡA produced concentration-dependented antiproliferative effect on SMMC-7721 cells at all experimental concentrations.Enhanced antiproliferative effects were obtained when SMMC-7721 cells were cultured with tanshinone ⅡA at 1.0,2.0,5.0,10.0 mg·L^-1 combined with 1.0,2.0,5.0 mg·L^-1 oxaliplatin(q>1.15).FCM results indicated that the apoptosis was induced effectively by both tanshinone ⅡA and oxaliplatin in SMMC-7721 cell,and synergic effect at above-mentioned concentrations was found in inducing SMMC-7721 cells apoptosis.CONCLUSION Tanshinone ⅡA can significantly inhibit the growth of SMMC-7721 cells,and enhance its oxaliplatin sensitivity,the mechanism may be associated with the synergic effect of tanshinone ⅡA and oxaliplatin in inducing apoptosis.