PCR-荧光探针法检测分枝杆菌的临床应用价值

目的 比较PCR-荧光探针法和改良罗氏培养法检测结核分枝杆菌和非结核分枝杆菌的一致性,探讨两种方法在临床上的应用价值.方法 应用PCR-荧光探针法检测69例改良罗氏培养阳性的分枝杆菌核酸,比较两种方法检测结果的一致率;两种方法检测结果不一致样本进行测序验证,以测序结果作为金标准,分析两种方法检测结果的正确率及不一致的可能原因.结果 两种方法检测总一致率为89.9%,结核分枝杆菌检测一致率为87.5%,非结核分枝杆菌检测一致率为90.6%.PCR-荧光探针法和改良罗氏培养法共有7例检测结果不一致,与测序结果比对后,改良罗氏培养法检测的正确率为94.2%,PCR-荧光探针法检测的正确率为92.8%.结论 操作简单、快速,灵敏的PCR-荧光探针法与作为传统金标准的改良罗氏培养法检测结核分枝杆菌和非结核分枝杆菌的一致性良好、正确率高,可作为临床分枝杆菌感染诊断的重要检测手段.

Clinical Value of PCR-fluorescence probe of mycobacterium

Objective To compare the consistency of mycobacterium tuberculosis and non-tuberculous mycobacteria detected by PCR-fluorescence probe and Lowenstein-Jensen culture,then explore the value of two methods clinical ap- plication. Methods 69 cases of Lowenstein Jensen culture positive mycobacterial nucleic acid were detected by PCR- fluorescent probe to compare and analyse the consistency rate of two results and the possible reasons of incongruity re- sults which would be verified by sequencing which is believed as the gold standard. Results The total concordance rate of two detecting methods was 89.9 , mycobacterium tuberculosis concordance rate was 87.5 , non-tuberculous myco- bacteria was 90.6 . Comparing 7 cases of incongruity with sequencing results, Lowenstein-Jensen culture precision rate was 94.20/oo ,PCR-fluorescenee probe was 92.8. Conclusion PCR-fluorescence probe which is easily operation .rapid and sensitivity and Lowenstein-Jensen culture which is considered as traditional gold standard have good consistency and high accuracy,so two methods can be regarded as an important method in clinical diagnosis of mycobacterial infec- tion.