神经肽Y在人垂体腺瘤中组织学和分子生物学变化的研究

目的 探讨NPY在人垂体腺瘤细胞中的位置和表达,不同类型中的特点.方法 收集我院神经外科经手术和病理确诊的垂体腺瘤57例.免疫电镜研究NPY在垂体腺瘤细胞中的位置.免疫组化和RT-PCR检测NPY的表达.结果 (1)免疫电镜:NPY主要位于胞质的分泌颗粒中,其次位于粗面内质网和细胞基质中.(2)免疫组化:NPY在垂体腺瘤中总体表达的结果为59.6%(34/57);促性腺瘤91.7%,GH腺瘤和ACTH腺瘤均为66.7%,零细胞腺瘤和混合性腺瘤均为50%,PRL腺瘤为25%,它们间的差异有统计学意义(P=0.04).(3)RT-PCR:NPY mRNA在垂体腺瘤中均表达,其差异有统计学意义(P<0.01);促性腺瘤最高是PRL腺瘤的9.3倍、无功能腺瘤5.8倍、GH腺瘤2.7倍,其余组间的差异无统计学意义.结论 (1)人垂体腺瘤中存在NPY.(2)垂体腺瘤中的确有NPY表达,不同类型中的表达各有差异;促性腺瘤表达最高,PRL腺瘤表达最低.(3)为今后深入探讨NPY在垂体腺瘤中的作用奠定了基础.

Study on chang of histopathology and molecular biology of NPY in human pituitary adenomas

Objective To investigate the localization of neuropeptide Y(NPY) in the subcellular structure and expression of NPY in human pituitary adenomas, and their specificity and relationship. Method The tissues of pituitary adenomas were obtained from 57 patients with pituitary adenomas who underwent surgery in the department of neurosurgery in General hospital of Tianjin Medical University. The localization of NPY in pituitary adenoma cells was studied by immuno-electron microscopy. The expression of NPY in all patients with pituitary adenomas were studied by immunohischemical analysis and RT-PCR. Results (1)Immuno-electron microscopy demonstrated that the positive NPY was located mainly in the secretory granulas in cell matrix of pituitary adenomas. Gold particles were sparsely detected in the rough endoplasmic reticulum and cell matrix. (2) Immunohistochemical analysis showed that NPY was immunodetected in 59.6% (34/57) patients with pituitary adenomas. NPY expression was significantly different among different types of pituitary adenomass (P<0.05). NPY was immunodetected in 91.7% of gonadotrophic adenomas, 66.7% of GH and ACTH adenomas, 50% of null cell adenoma and plurihormonal adenoma, 25% of prolaetinomas. There was significant difference among them(χ~2=11.64, P=0.04). (3) RT-PCR showed that there was signification differentiation (F=4.21, P<0.01) among the expression of NPY in different types of pituitary adenoma. The NPY expression in gonadotrophic adenomas was the highest (7.00±8.32),with 9.3-fold(q=5.2616, P<0.01)compared with prolactinomas(0.75±0.11),5.8-fold (q=5.0143, P<0.01) with non-thnctional adenomas (1.79±2.13),2.7-fold (q=4.07, P<0.05) with GH adenomas (2.59±3.06). There was no significant differentiation among the rest groups. Conclusions (1)NPY really exists in human pituitary adenomas. (2)NPY expression is significantly different among different types of pituitary adenomas. The NPY expression level in gonadotrophic adenomas is the highest, the lowest in prolactinomas. (3)These results will provide sound bases for further research of the action of NPY in human pituitary.