应用组织工程方法构建瘢痕疙瘩模型

目的 应用组织工程方法构建人瘢痕疙瘩动物模型,并探讨采用这一模型进行瘢痕疙瘩临床和实验室研究的可行性。方法 对人体瘢痕疙瘩组织中成纤维细胞进行原代培养,将体外培养第6~8代的成纤维细胞,接种到培养液预湿的聚乳酸-乙醇酸共聚物(PLGA)支架,形成体外复合体,将复合体转移至旋转式细胞培养系统容器内培养;1周后种植在20只雌性裸鼠皮下,同体两侧对照,第4、8、16、24周取材,对获得的瘢痕疙瘩组织进行组织学评价。结果 术后裸鼠全部成活。复合体移植24w后,在裸鼠皮下形成的瘢痕疙瘩保持了其原有的胶原形态,在电镜下可观察到植入物内同时存在纤维细胞和成纤维细胞,成纤维细胞内可见丰富的粗面内质网,细胞特性保持不变。结论 PLGA与瘢痕疙瘩成纤维细胞具有较好的亲和性,PLGA-瘢痕疙瘩成纤维细胞复合体在裸鼠体内可形成瘢痕疙瘩,值得进一步开发研制。

Construction of animal models of keloid by tissue engineering

s:Objective To construct animal model of keloid by using tissue engineering and to explore its potential clinical significance.Method Fibroblasts from keloid were isolated and cultured.After being cultured to generation six or eight,fibroblasts were inoculated into scaffolds constructed from copolymers of polylactic acid (PLA) and polyglycolic acid (PGA),and then cultured in rotatory cell culture system for 1 week prior to subcutaneous transplantation into 20 athymic mice.After 4,8,16,24 weeks,the specimens were cut out and examined histologically.Results All the mice survived after surgery.The collagen patterns of all the keloids were remained after 24 weeks.A lot of fibroblasts and their secreted collagen were observed under light microscope.Enlarged rough endoplasmic reticula inside the fibroblasts were revealed by transmission electron microscopy.The fibroblasts in specimens remained the capability of synthesizing and secreting collagen.Conclusions There is a good affinity between PLA /PGA and fibroblasts.Complex of PLA /PGA and fibroblasts could be cultured into keloids in athymic mice.