| 反相高效液相色谱法测定血浆和脑脊液中甲氨蝶呤浓度 |
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| 引用本文： | 吴闯,杨正鸿,林贵业,陆泰远.反相高效液相色谱法测定血浆和脑脊液中甲氨蝶呤浓度[J].中国医院药学杂志,1999,19(5):6. |
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| 作者姓名： | 吴闯 杨正鸿 林贵业 陆泰远 |
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| 作者单位： | 1. 广西医科大学第一附属医院,南宁530027
2. 广西桂林医学院 |
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| 摘 要： | 目的：建立测定血浆和脑脊液中甲氨蝶呤的反相高效液相色谱方法。方法：采用ＯＤＳ不锈钢柱，流动相：甲醇∶０．０２５ｍｏｌ·Ｌ－１磷酸盐缓冲液（２１∶７９，ｐＨ６．７０），检测波长：３０６ｎｍ，样品经沉淀蛋白质后直接进样。结果：血浆和脑脊液样品回收率分别为９７．５％±４．１％和１００．８％±１．５％，日内和日间误差（ＲＳＤ）均＜７％，最低检测浓度４．７５×１０－８ｍｏｌ·Ｌ－１，血浆中在１．１０×１０－７～２．２×１０－６ｍｏｌ·Ｌ－１和２．２０×１０－６～１．７６×１０－４ｍｏｌ·Ｌ－１；脑脊液中在１．１０×１０－７～２．２０×１０－６ｍｏｌ·Ｌ－１和２．２０×１０－６～１．１０×１０－３ｍｏｌ·Ｌ－１浓度范围内有良好线性关系，ｒ＝０．９９９６～０．９９９９。结论：本法简便、准确、灵敏。
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| 关 键 词： | 甲氨蝶呤，反相高效液相色谱法，药物浓度 |
Determination of methotrexate level in plasma and cereberospinal fluid by HPLC |
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| Wu Chuang,Yang Zhenhong,Lin Guiyi,et al.Determination of methotrexate level in plasma and cereberospinal fluid by HPLC[J].Chinese Journal of Hospital Pharmacy,1999,19(5):6. |
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| Authors: | Wu Chuang Yang Zhenhong Lin Guiyi |
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| Abstract: | OBJECTIVE :To establish an HPLC method for the determination of methotrexate level in plasma and cereberospinal fluid (CSF). METHODS :The separation was performed in a Shimpack CLCC18 column (15cm6.0mm ID) with a mobile phase of methanol:0.025 molL-1,phosphate buffer (2179,pH6.70) and determinated at 306 nm.RESULTS :The measurable lowest concentration was 4.7510-8 molL-1.The good linear relationship between peak and methotrexate concentration in plasma and in CFS was found.The experiment showed that the recovery from plasma and CSF were 97.5%4.1% and 100.8%1.5%,respectively.The RSD(%) of within day and betweenday were less than 7%. CONCLUSIONS :The method is simple,accurate. |
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| Keywords: | methotrexate HPLC drug concentration |
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