槲皮素与羟丙基-β-环糊精包合物的药物动力学研究

建立了血浆中槲皮素-羟丙基-β-环糊精包合物的HPLC分析方法,并用此方法对大鼠静脉注射及灌胃给药进行药物动力学研究,血浆标本经甲醇-冰醋酸提取,以乙腈-水为流动相,紫外360 nm处检测,槲皮素的线性范围在1 μg/ml～150 μg/ml,最低检测浓度为0.5 μg/ml.槲皮素在大鼠体内的药物动力学过程均符合二室开放模型,其中包合物灌胃时t1/2(β)＝2.220 h,静脉注射时t1/2(β)＝90.871 min.

Pharmacokinetic Studies of Quercetin-hydroxypropyl-β-cyclodextrin Inclusion Compound in Rats

To estabilish a HPLC method for determination of quercetin-hydroxypropyl-beta-cyclodextrin in rats plasma and investigate the pharmacokinetics characteristics of it in rats. The plasma was extracted with methanol-aceitc acid (9:1). The mobile phase was acetonitrilewater. Absorbance of the effluence was monitored at 360 nm. The linear limit of quercetin was within the range of 1 to 150 microg/ml. The lowest limit of quercetin was 0.5 microg/ml. The concentration-time curve of quercetin in rats was considered of a two-compartment open model, and the t1/2 (beta) of the inclusion compound when ig is 2.220 h, the t1/2 (beta) of the inclusion compound when iv is 90.871 min.