大孔吸附树脂分离纯化丹皮总苷工艺研究

目的:优选大孔吸附树脂富集纯化牡丹皮中丹皮总苷的最佳工艺参数。方法:采用HPLC法测定丹皮总苷含量,考察D101、D201、D301、D401、AB-8、NKA-96种大孔树脂对芍药苷的吸附和解吸附性能,并进一步考察分离纯化条件。结果:D101大孔树脂对丹皮总苷提取液纯化最优,上样液芍药苷浓度为0.83mg·mL^-1、pH6.5、洗脱流速1BV·h^-1,分离纯化条件为:先用10BV蒸馏水洗脱,然后用4BV70%乙醇洗脱,收集70%乙醇洗脱液,浓缩、干燥,即得到丹皮总苷。结论:D101大孔树脂可用于牡丹皮水提取液中丹皮总苷的富集纯化。

Study on Separation and Purification Total Glycosides of Tree Peony Bark by Macroporous Resin

Objective:To optimize the technical parameters of enrichment and purification of the total glycosides in tree peony bark with macroporous resin. Methods:HPLC method was used to analyze the content of total glycosides,the adsorption and desorption performance of D101,D201,D301,D401,AB-8,NKA-9 were investigated,and the purification of conditions were inspected. Results:The best macroporous resin for purified extractive of total glycosides in tree peony bark was D101,the concentration of Paeoniflorin was 1.83 mg·mL^-1 with pH 6.5,and with the elution flow rate of 1 BV·h^-1. Purification of the conditions were as follows:10 BV water and 4 BV 70% ethanol were used as eluant of paeoniflorin,respectively. Portions of 70% ethanol were collected,concentrated,and dried. Conclusion:D101 resin can be used to refine the total glycosides in the water extracts of tree peony bark.