Immimological titration of 3-N-acetyl-hydroxyamino-4, 6-dimethyldipyrido(l, 2-a: 3', 2'-d)imidazole-rat liver DNA adducts

Antibodies to N-(guanosin-8-yI)-3-N-acetylamino-4, 6-di-methyldipyrido(l,2-a:3', 2'-d)imidazole were elicited in rabbits by immunizationwith a conjugate formed between this compound and bovine serumalbumin. The specificity of the antibodies was studied by radioimmunoassay.These antibodies were used to titrate the adducts formed inliver DNA of rats treated with 3-N-acetyl-hyoYoxyamino-4, 6-dimethyldipyrido,2-a: 3', 2'-d)iinidazole, a supposed metabolite of the mutagenicamine 3-amino-4, 6-dimethyldipyrido(l, 2-a:3', 2'-d)imidazofe(Glu-P-3). It is found that Gtu-P-3 residues are covalentlybound to the C8 of guanine in acetylated form (70%) and deacetylatedform (30%).