都匀亚洲牛带绦虫病小鼠肝纤维化肝脏CTCF、TCF-β1的表达意义

目的研究结缔组织生长因子（connective tissue growth factor,CTGF）与转化生长因子-β1（transforming growthfactor-betal,TGF-β1）在都匀亚洲牛带绦虫小鼠肝纤维化肝脏中的表达状况及意义。方法用昆明小鼠感染都匀亚洲牛带绦虫建立小鼠肝纤维化模型;感染小鼠于30、40、50、60 d剖杀,HE染色观察肝脏病理变化,免疫组化法检测肝内CTGF、TGF-β1蛋白的表达水平。结果感染小鼠30 d时形成都匀亚洲牛带绦虫肝纤维化,40d时肝内CTGF和TGF-β1蛋白表达达高峰,随后出现下降,感染小鼠CTGF和TGF-β1蛋白表达水平具有直线相关性。结论CTGF与TGF-β1的表达与都匀亚洲牛带绦虫小鼠肝纤维化形成有密切关系,TGF-β1的致纤维化作用可能部分通过CTGF的生物学作用介导,阻断CTGF的传导通路可能是绦虫病肝纤维化治疗的有效靶点。

The expression and significance of CTCF and TCF-β1 in murine liver fibrosis induced by Taenia Saginata Asiatic from Duyun area

Objective：To study the expression and significance of connective tissue growth factor （CTGF） and transforming growth factor-batal （TGFβ1） in murine liver fibrosis induced by Taenia saginata Asiatic from Duyun area. Methods： Establish the murine liver fibrosis model induced by Taenia saginata Asiatic from Duyun area, and subsequently killed at the 30^th,40^th,50^th. and 60^th days respectively. The hepatic expression of CTGF and TGF-β1 was detected by immunohistochemical method. Histological changes of murine liver were observed by HE staining. Results： The routine liver fibrosis model induced by Taenia saginata Asiatic from Duyun area were successfully established at the 30^th day. The hepatic expression of CTGF and TGF-β1, in the murine liver fibrosis models peaked at the 40^th day and then declined. There existed positive correlation between the hepatic expression of CTGF and TGF-β1. Conclusion： The expression of CTGF and TGF-β1 is closely correlated to the development of the murine liver fibrosis induced by Taenia saginata Asiatic from Duyun area, and TGF-β1 may play its fibering roles by roles action on CTGF. To block the CTGF signal transduction pathway may be a potential treatment of Taenia Saginata Asiatic liver fibrosis.