丹红注射液及其活性组分对脂多糖诱导的小胶质细胞NO分泌的抑制作用

目的 研究丹红注射液及其单体成分对小鼠小胶质细胞株(BV-2细胞)的影响及其作用机制.方法 实验分为对照组、脂多糖(LPS)刺激组、加药组,检测不同稀释倍数的丹红注射液、水和醋酸乙酯萃取物及其单体成分对小胶质细胞活力及LPS诱导小胶质细胞一氧化氮(NO)产生的影响.结果 丹红注射液及醋酸乙酯萃取物对LPS诱导的小胶质细胞NO的产生没有抑制作用,但是丹红注射液水萃取物以及丹酚酸A可以在不影响细胞活力的情况下抑制NO产生.结论 丹红注射液水萃取物中的某些成分以及丹酚酸A抑制激活小胶质细胞NO的产生可能是丹红注射液神经保护作用的机制之一.

nhibition of Danhong Injection and its active components on NO secretion in microglia induced by lipopolysaccharide

Objective To study the effect and its mechanism of action of Danhong Injection and its monomer components on microglia BV-2 of mice. Methods The experiment was divided into control, lipopolysaccharide (LPS), and dosing groups. The effects of Danhong Injection, water extract, ethyl acetate extract, and monomer compounds on microglia cell viability and LPS-induced NO production were detected. Results Danhong Injection and its ethyl acetate extract had no inhibitory effect on NO production of LPS-induced microglia, but water extract of Danhong Injection and its active component salvianolic acid A affected NO production without affecting cell viability. Conclusion The monomer components from Danhong Injection and salvianolic acid A inhibition NO production in activating microglia may be one of the neuroprotective effect mechanisms for neuroprotection of Danhong Injection.