解析COMT基因Met多态与子宫内膜异位症的关系

目的分析COMT基因Met多态与子宫内膜异位症的关系。方法选取2011年6月至2012年6月期间于我院就诊的100内膜异位症患者为试验组(其中包括45腺肌病患者),以蛋白酶K消化-饱和酚氯仿法对其外周血细胞基因组DNA进行提取,并采用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)方法对以上患者进行基因分型,另选取同时期100例未患有子宫内膜异位症(其中包括50例非腺肌病患者)的患者为对照组,采取与试验组同样的方式进行基因分型。结果对比两组G/G、G/A、A/A基因型频率,其中试验组分别为38.0%、37.0%、25.0%,对照组分别为40.0%、41.0%、19.0%,两组对比差异性较小,无统计学意,P>0.05;对比两组A等位基因频率,试验组为42.3%,对照组为34.1%,两组对比差异性较小,无统计学意义,P>0.05。结论 COMT基因Met多态与子宫内膜异位症无明显相关性。

Analysis of the COMT Gene Met Polymorphism with Endometriosis Relationship

Objective Analysis of the COMT gene Met polymorphism with endometriosis relationship. Methods From 2011 June to 2012 June in our hospital during the period 100 endometriosis patients into test group （including 45 patients with adenomyosis）, with proteinase K digestion - saturated phenol chloroform method on peripheral blood cell genomic DNA extraction, and polymerase chain reaction - restriction fragment length polymorphism （ PCR-RFLP method for more patients ） were genotyped, another takes the same period 100 patients did not suffer from endometriosis （ including 50 cases of patients with adenomyosis ） patients as control group and test group, taking the same way of genotyping. Results Comparison of the two groups of G/G, G/A, A/A genotype frequency, wherein the test group were 38%,37%,25%, the control group were 40%, 41%,19%, two groups of contrast difference is small, no statistical significance, P〉0.05;in contrast to the two group of A allele frequencies, experiment group 42.3%,control group for 34.1%, a smaller difference between the two groups, no statistical significance, P〉0.05. Conclusions The COMT gene Met polymorphism with endometriosis without obvious correlation.