1,2-二氯乙烷对血脑屏障的损伤作用

目的 探讨1,2-二氯乙烷对血脑屏障的损伤作用.方法 用1,2-二氯乙烷静式呼吸道急性染毒复制大鼠急性中毒性脑病模型,用硝酸镧染色法测定脑组织的含水量并检测血脑屏障的通透性;同时在体外培养脑微血管内皮细胞和神经胶质细胞,用1,2-二氯乙烷对生长良好的脑微血管内皮细胞和神经胶质细胞染毒,在光镜和电镜下观察细胞形态学特征.结果 （1）脑组织主要表现为细胞外水肿,镧示踪法检测到血脑屏障有镧颗粒的大量渗漏.（2）中高剂量染毒组大鼠大脑皮质含水量较对照组明显升高,并且随着染毒后时间的延长更趋严重;大脑髓质含水量仅在染毒后观察6 h组有统计学意义（P＜0.05）.（3）1,2-二氯乙烷能破坏脑微血管内皮细胞和神经胶质细胞的正常形态学结构,且对神经胶质细胞的损伤表现得较早、较严重.结论 1,2-二氯乙烷可造成血脑屏障的损伤,引起血管源性脑水肿.

Effect of 1,2-dichloroethane on blood brain barrier

Objective To investigate the effect of 1,2-dichloroethane(1,2-DCE)on blood brain barrier. Methods Acute toxic encephalopathy model was copied with the consecutive static inhalation of 1,2-DCE.The water content of brain tissue was measured,and the blood brain barrier permeability was detected with lanthanum nitrate.The brain microvascular endothelial cells and neuroglial cells were cultured in vitro,which were administrat- ed with 1,2-DCE.The cell morphologic structures were observed under light microscope and electron microscope. Results (1)The extracellular edema was most found in the cerehral tissue and the leakage of lanthanum particles through the barrier were found with the lanthanum tracking method.(2)The water content in cerebral cortex in the moderate and high dose groups was significantly higher than that in the control group and became severer with the increases of the intoxicated time.The water content in cerebral medulla was significantly increased only at 6 hours after the intoxication.(3)The normal morphological structure of brain microvascular endothelial cells and neuroglial cells could be injured by 1,2-DCE,And the injury to neuroglial cells caused by 1,2-DCE occurred earlier and sev- erer than that to brain microvascular endothelial cells.Conclusion 1,2-DCE can damage blood brain barrier and induce cerebral edema.