红树植物角果木内生真菌J62生物活性成分研究

目的 研究红树植物角果木内生真菌J62产生的活性次生代谢产物。方法 采用多种柱色谱技术进行分离纯化,根据理化常数和波谱数据鉴定化合物的结构,采用滤纸片琼脂扩散法测定化合物抗金黄色葡萄球菌(Staphylococcus aureus)和白色念珠菌(Candida albicans)活性,利用MTT法测定各化合物的体外细胞毒活性。 结果 从角果木内生真菌J62发酵液中分离鉴定了10个化合物,分别为：反式桂皮酸 (1),3,4-二甲氧基苯甲醇 (2), 2-[(3,4-dimethoxyphenyl)methyl]-4,5-dimethoxy-benzenemethanol (3), l尿嘧啶核苷(4), similin B (5),coibanol C (6), hymatoxin C (7), 5,8-epidioxy-5a,8a-ergosta-6,22E-dien-3b-ol (8),豆甾-7,22-二烯-3b,5a,6a-三醇 (9)和crispin A (10)。结论 生物活性测试结果表明,化合物1, 2和7具有抗金黄色葡萄球菌活性,化合物1和2具有抗白色念珠菌的活性；化合物2和5对肿瘤细胞株K-562显示有生长抑制活性。

Bioactive compounds from the endophytic fungus J62 of mangrove plant Ceriops tagal

Ten compounds were isolated and purified from the fermentation broth of endophytic fungus strain J62 of mangrove plant Ceriops tagal by means of chromatographic technology. Their chemical structures were identified as trans-cinnamicacid (1), 3,4-dimethoxybenzyl alcohol (2), 2-[(3,4-dimethoxyphenyl)methyl]-4,5-dimethoxy-benzenemethanol (3), uridine (4), similin B (5), coibanol C (6), hymatoxin C (7), 5,8-epidioxy-5a,8a-ergosta-6,22E-dien-3b-ol (8), stigmasta-7,22-diene-3b,5a,6a- triol (9), and crispin A (10) on the basis of spectral data. Compounds 1, 2, and 7 showed inhibitory effect on Staphylococcus aureus, and compounds 1 and 2 expressed inhibitory effect on Candida albicans by paper disc diffusion method. Compounds 2 and 5 showed inhibition effect on the cell lines of K-562 by MTT method.