斛皮素缓解阿霉素急性肝脏毒性的实验研究

目的]研究斛皮素对阿霉素引起的小鼠急性肝脏毒性的作用。方法]C57BL/6小鼠随机分成4组：对照组、斛皮素组、阿霉素组、联用组,观察体重、血清谷草转氨酶（AST）、谷丙转氨酶（ALT）变化,HE染色观察组织病理学改变,TUNEL染色观察细胞凋亡情况。结果]阿霉素组小鼠死亡率为20%,其余组为0。斛皮素能明显缓解阿霉素引起的体重减轻及AST、ALT酶系失调,并明显缓解肝脏细胞的病变及细胞凋亡的发生。结论]斛皮素对阿霉素引起的小鼠急性肝脏毒性具有保护作用。

Experimantal Study on Quercetin Relieving Acute Hepatotoxicity Induced by Doxorubicin

Purpose] To investigate the effect of quercetin on doxorubicin （Dox） induced acute hepatotoxicity. Methods] C57BL/6 mice were randomly divided into 4 groups ：control group, quercetin group,Dox group,and combined group. Body weight was detected everyday. The serum contents of ALT and AST were detected. The pathological changes of liver tissues were observed by HE staining. The apoptotic cells were detected by TUNEL staining. Results] The mortality of Dox group was 20%,while it was zero in other 3 groups. Quercetin pretreatment significantly relieved Dox-induced loss of weight and disorder of serum levels of ALT and AST. Consistently, quercetin reversed the Dox-induced pathological changes and apoptosis in liver tissues of mice. Conclusion] Quercetin has protective effect on Dox-induced acute hepatotoxicity.