芩丹胶囊对自发性高血压大鼠主动脉损害的保护和逆转作用

目的 观察芩丹胶囊对自发性高血压大鼠（SHR）主动脉结构的影响并探讨其可能的机制。方法 将14周龄SHR分为芩丹胶囊组、牛黄降压胶囊组、卡托普利组和模型组，Wistar-Kyoto（WKY）大鼠作为正常对照组，分别给予相应的药物并测量血压。治疗12周后，HE和Masson染色观察主动脉形态学变化，放免法检测主动脉血管紧张素Ⅱ（Ang-Ⅱ）含量，荧光实时定量PCR检测动脉壁碱性成纤维细胞生长因子（bFGF）mRNA表达水平。结果芩丹胶囊具有明显降压作用，能够改善SHR主动脉结构，降低血管组织Ang-Ⅱ含量，并抑制主动脉壁bFGFmRNA的表达（P〈0．05或P〈0．01），疗效与卡托普利类似，且与牛黄降压胶囊比较差异有显著性（P〈0．01）。结论 芩丹胶囊对SHR主动脉损害具有明显的保护和逆转作用，其机制可能与降低局部AngⅡ含量，抑制血管壁bFGF mRNA的表达有关。

Protective and Reverse Effects of Qindan Capsule on Aortic Lesion in Spontaneously Hypertensive Rats

OBJECTIVE: To investigate the effects and mechanism of Qindan Capsule (QC) on aortic structure in spontaneously hypertensive rats (SHR). METHODS: Thirty-two SHR of 14 weeks old, were divided into the QC group, the Niuhuang Jiangya Capsule (NJC) group, the captopril group and the model group. Besides, Wistar-Kyoto (WKY) rats were taken as the normal control. All the others were administered with corresponding medicine and their blood pressure measured. After 12 weeks, the morphological changes of aorta were observed by HE and Masson staining, the level of angiotensin II (Ang II) in aorta was detected by radioimmunoassay, and the mRNA expression of basic fibroblast growth factor (bFGF) in aortic wall was analyzed by real-time quantitative fluorescent PCR. RESULTS: QC could reduce the blood pressure in SHR, improve their aortic structure, lower the Ang II level and inhibit the bFGF mRNA expression in aortic wall (P < 0.05 or P < 0.01), showing a good effect similar to that of captopril (P > 0.05) and better than that of NJC (P < 0.01). CONCLUSION: QC has a significant protective and reverse effect on aortic lesion in SHR. The mechanism may be related to its actions in reducing Ang II level and inhibiting bFGF mRNA expresion in aortic wall.