通脉宁调控AngⅡ、LPC诱导血管平滑肌细胞c-fos、c-myc基因表达的研究

目的观察通脉宁胶囊对血管紧张素Ⅱ(AngⅡ)、溶血磷脂酰胆碱(LPC)诱导的兔血管平滑肌细胞(VSMC)c-fos、c-myc癌基因表达的影响。方法组织贴块法培养兔主动脉平滑肌细胞,用AngⅡ、LPC诱导VSMC增殖,采用RT-PCR方法,观察通脉宁全方及益气拆方和活血拆方药物血清对AngⅡ、LPC诱导的VSMC c-fos、c-myc蛋白表达。结果AngⅡ、LPC组c-fos和c-myc蛋白表达增加,与空白组比较有显著性差异(P<0.01);通脉宁全方组、益气拆方组、活血拆方组均可使c-fos和c-myc的蛋白表达下调,与AngⅡ、LPC组比较有显著性差异(P<0.01);通脉宁全方组与益气拆方组、活血拆方组比较也有显著性差异(P<0.01)。结论通脉宁胶囊可下调AngⅡ、LPC诱导的VSMC c-fos、c-myc基因表达,而对VSMC增殖起到抑制作用。

Regulative effect of Tongmaining Capsules on expressions of c-fos and c-myc genes in VSMC induced by AngII and LPC

Objective To observe the influence of Tongmaining Capsules on the expressions of cancer genes,c-fos and c-myc,in rabbit vascular smooth muscle cells(VSMC) induced by angiotensin II(AngII) and lysophosphatidylcholine(LPC).Method To culture rabbit VSMC by tissue-sticking method and than to induce the proliferation of VSMC by AngII and LPC.To use RT-PCR to observe the influences of the serum of Tongmaining Capsules,its decompositions with the effect of replenishing qi or the effect of activating blood circulation,on the expressions of c-fos and c-myc genes in VSMC.Result The expressions of c-fos and c-myc genes were increased in the AngII group and the LPC group with a significant difference compared with that in the blank group(P<0.01).In the group of Tongmaining Capsules and groups of decompositions with the effect of replenishing qi or the effect of activating blood circulation the expressions of c-fos and c-myc genes were decreased with a significant difference compared with that of the AngII group and LPC group(P<0.01). There was also a significant difference among above three groups(P<0.01).Conclusion Tongmaining Capsules has a down-regulative effect on the expressions of c-fos and c-myc genes in VSMC induced by AngII and LPC,therefore,it can inhibit the proliferation of VSMC.