| SHG－44细胞显微注射NDGA诱导分化的初步观察 |
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| 引用本文： | 卞修武,史景泉,傅晓岚,施同舟,谭骏,刘昕.SHG－44细胞显微注射NDGA诱导分化的初步观察[J].临床与实验病理学杂志,1995(1). |
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| 作者姓名： | 卞修武 史景泉 傅晓岚 施同舟 谭骏 刘昕 |
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| 作者单位： | 第三军医大学病理学教研室，重庆中心实验室，重庆分子生物学教研室 |
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| 摘 要： | 采用显微注射系统对ＳＨＧ－４４细胞进行单细胞微量注射技术的探讨，并观察去甲二氢愈创木酸（ＮＤＧＡ）注射后的作用。结果显示，体积较小的肿瘤细胞显微注射成功的关键在于注射参数的正确设定、细胞标记、注射针下限值的确定、注射过程中防污染以及操作细致、迅速；ＮＤＧＡ注射ＳＨＧ－４４细胞后的诱导分化作用较其在细胞外培养基中的作用更直接、迅速和显著，亦更持久。
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| 关 键 词： | 胶质瘤，显微注射，分化，去甲二氢愈创木酸 |
The preliminary observation of differentiation induced by microinjection of nordihydroguaiaretic acid in SHG-44 cell line |
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| Bian Xiuwu,Shi Jingquan,Fu Xiaolan,et al.The preliminary observation of differentiation induced by microinjection of nordihydroguaiaretic acid in SHG-44 cell line[J].Chinese Journal of Clinical and Experimental Pathology,1995(1). |
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| Authors: | Bian Xiuwu Shi Jingquan Fu Xiaolan |
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| Abstract: | A new microinjection system was used to investigate the technology of single cell microinjection in SHG-44 cell line and the effect of differentiation induced by nordihy droguaiaretic acid(NDGA).The result showed that success of the injection into the small tumor cells depended on correct parameters of injection,cell marking, proper low limit of the tip. prevention of pollution ring mection,as well as careful and rapid operation. The present study also revealed the rapid,direct,obvious and permanent effect of differentiation induced by this direct injection of NDGA. |
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| Keywords: | glioma cell microinjection differentiation nordihydroguaiaretic acid |
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