内生真菌菌丝及代谢物对铁皮石斛及金线莲生长的影响

目的 研究开唇兰小菇、石斛小菇和兰小菇3种小菇属内生真菌菌丝体及代谢物对兰科药用植物铁皮石斛及金线莲生长的促进作用。方法 在植物试管苗培养基中分别加入20%（V/V）真菌菌丝及10mg/L发酵液的乙酸乙酯提取物,观察对细胞增殖及苗生长的影响。结果 3种菌的菌丝体及兰小菇的乙酸乙酯提取物能显著提高铁皮石斛原球茎的增殖率;石斛小菇的菌丝体对金线莲的生长和侧芽增殖有显著促进作用;开唇兰小菇和兰小菇的乙酸乙酯提取物分别对增加金线莲侧芽发生数及生长有显著促进作用。结论 3种内生真菌对铁皮石斛、金线莲的促生长作用与菌丝内及分泌到菌丝外的代谢产物有关。

Effects of endophytic fungal hyphae and their metabolites on the growth of Dendrobium candidum and Anoectochilus roxburghii

Objective To study the effects of hyphae and their metabolites of Mycena anoectochila,M.den-drobii and M.orchidicola on promoting the protocorns multiplication of D.candidum and platelet growth of A.roxburghii.Methods Add20%(V/V)ground hypha or10mg /L ethyl acetate extracts of fermentation liquid respectively to the plant culture media.Results The ground hypha of3Myce-na and ethyl acetate extracts of M.orchidicola could promote the protocorn multiplication rate of D.ca ndidum.The ground hypha of M.dendrobii could increase the numbers of bud and improve the platelet growth of A.roxburghii.The ethyl acetate extracts of fermentation liquid of M.anoectochila increased the numbers of bud and M.orchidicola significantly improved the platelet growth of A.rox-bu rghii.Conclusion The effects of3Mycena Endophytic fungi on promoting growth of Dendrobium candidum and Anoectochilus roxburghii are related with their metabolites in and out of hypha.