| 高效液相色谱法分离分析链霉素及其杂质 |
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| 引用本文： | 周玉 孔英梅. 高效液相色谱法分离分析链霉素及其杂质[J]. 中国抗生素杂志, 1990, 15(3): 175-179 |
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| 作者姓名： | 周玉 孔英梅 |
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| 作者单位： | 中国药品生物制品检定所 北京(周玉，孔英梅，唐秋瑾)，中国药品生物制品检定所 北京(郑昌亮) |
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| 摘 要： | 用离子对反相高效液相色谱法可从国内外商品链霉素中分离出包括剧毒的、致敏的及色素原部份等17种杂质,并可定量测定链霉素、链霉胍及链胍双氢链糖。由于此法系分离后再测定,故不受其他物质的干扰,较微生物法及化学比色法专属性高,且可用于测定原料、成品、发酵液及分离纯化各阶段的液体样品。
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| 关 键 词： | 链霉素 色谱法 杂质 分离 分析 |
SEPARATION AND DETERMINATION OF STREPTOMYCIN BY REVERSE PHASE HPLC |
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| Zhou Yu Kong Ying-mei Tang Qiu-jin and Zheng Chang-Hang. SEPARATION AND DETERMINATION OF STREPTOMYCIN BY REVERSE PHASE HPLC[J]. Chinese Journal of Antibiotics, 1990, 15(3): 175-179 |
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| Authors: | Zhou Yu Kong Ying-mei Tang Qiu-jin and Zheng Chang-Hang |
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| Abstract: | An isocratic, paired-ion reversed-phase HPLC method for the determination of streptomycin, streptidine and dihydrostreptose(1-4)streptidine has been developed. 17 components such as allergenic impurities, highly toxic impurities, st-reptobiosamine etc., in commercial streptomycin, could be separated by this method. The method employs a microparticulate reversed-phase column(Bondapak C13 or Du Pont ODS) and a mobile phase composed of 0.005 mol/L sodium hexanesulfonate, 0.1mol/L sodium dihydrogen phosphate and 0.0018 mol/L phosphoric acid in aceton-itrile-water (5:95v/v) at pH 3.8 with detection at UV 195nm. |
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| Keywords: | Streptomycin Reversed phase high performance liquid chromatography |
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