Doxorubicin release from self-assembled nanoparticles of deoxycholic acid-conjugated dextran |
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Authors: | Young-IL Jeong Kyu-Don Chung Ki Choon Choi |
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Institution: | (1) Department of Medicine, Tumor Hospital, Sun Yat-sen University, Guangzhou, China;(2) Department of Oral and Maxillofacial Surgery, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China |
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Abstract: | In this study, we synthesized deoxycholic acid (DA)-conjugated dextran (DexDA) and prepared doxorubicin (DOX)-encapsulated
nanoparticles using DexDA conjugates. Since DexDA conjugates have amphiphilic properties, they will show self-aggregation
behavior at aqueous environment. To approve self-aggregation behavior, critical aggregation concentration value of DexDA conjugates
was evaluated using fluorescence spectroscopy. DOX-incorporated DexDA nanoparticles were less than 200 nm. The higher substitution
degree of DA and higher drug feeding ratio resulted in increased particle size. Drug release was decreased by increase of
substitution degree value of DA and increase of drug feeding ratio. At in vitro cytotoxicity test using DOX-resistant CT26 colon carcinoma cells, higher antitumor activity was obtained with DOX-incorporated
nanoparticles compared to free DOX. Fluorescence microscopic observation verified this result, i.e. nanoparticles were properly
entered into tumors cells and maintained longer compared to DOX itself. These results suggested that DOX-incorporated DexDA
nanoparticles are promising vehicles for antitumor drug delivery. |
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