一枝黄花及其总黄酮总皂苷利尿作用的实验研究

目的：探讨一枝黄花煎剂及其总黄酮、总皂苷的利尿作用。方法：采用家兔输尿管法研究一枝黄花煎剂、总黄酮及总皂苷的利尿作用，并用原子吸收分光光度计测定家兔尿中Na^＋、K^＋浓度，比较用药前后尿量与离子的变化。结果：一枝黄花煎剂和总皂可以显著提高家兔排尿量（P〈0．01），产生较强的利尿作用，作用维持时间在2h以上；一枝黄花总黄酮可以明显提高家兔排尿量（P〈0.05），产生明显利尿作用，作用时间在2h以内。一枝黄花煎剂、总黄酮、总皂苷均可使单位容积尿液中的尿Na^＋、尿K^＋排出量减少（P〈0．05），表现为排水大于排钠排钾。结论：一枝黄花具有较强的利尿作用，一枝黄花总黄酮和总皂苷是一枝黄花发挥利尿作用的有效成分，且两成分之间在利尿作用方面可能有协同作用。一枝黄花及其总黄酮、总皂苷均可作为保钾利尿药使用。

Experimental Study on Diuretic Effects of Goldenrod Herb and Its Total Flavonoid and Total Saponins

Objective： To investigate the diuretic effects of Goldenrod Herb and its total tlavonoid and total saponins.Methods： The ureter collecting test in rabbits and the determination of the eoneertration of Na ^＋ , K ^＋ in urine by Atomic Absorption speetrophotometer were： used to study the diuretic e_fleets of Goldenrod Herb and its total tlavonoid and total saponin.Results： The urine volume in rabbits was significanfly increased by Goldenrod Herb decoction and its total saponin （ P 〈 0.01 ）, the strong diuretic effects would last at least 2 hours; The total flavonoid in Goldenrod Herb remarkably increased the urine volume in rabbits （ P 〈0.05）, and the diuretic effects would last less than 2 hours. Goldenrod Herb and its total flavanoid and total saponln could reduce the concentration of Na^ ＋ , K ^＋ in urine （ P 〈0.05） .Conclusion： The total flavonoid and total saponin were the effective ingredients of Goldenrod Herb, which has a strong diuretic effect.In addition, the total tlavonoid and total saponin might have a synergistic effect.Goldenrod Herb and its total flavonoid and total sapanin could be used as isokalaemie diuretics.