电离辐射后小鼠骨髓组成中结合珠蛋白的表达规律研究

目的研究照射前后结合珠蛋白在小鼠骨髓中的表达和分布，及其与骨髓细胞周期进程改变及凋亡的可能关联。方法用RT-PCR，Western blotting及流式细胞术的方法检测骨髓细胞Hp mRNA及细胞内外Hp蛋白的存在，及其辐射后的变化。用流式细胞术的方法分析了骨髓细胞中Hp阴性及阳性细胞群周期分布及凋亡率的差异。结果在骨髓细胞中检测到Hp mRNA的存在，而且8Gy照射后24h较正常小鼠有明显升高。骨髓细胞内及外液中检测到Hp蛋白的存在，同样8Gy照射后24h较正常小鼠亦呈明显升高。流式细胞术检测发现小鼠骨髓细胞中部分细胞含有Hp蛋白，照射后Hp阳性细胞的比例逐渐增加，并有着较好的时效关系和剂量依赖关系。骨髓Hp阳性细胞与阴性细胞的细胞周期分布存在显著的差异。在照射后6h骨髓细胞凋亡率随照射剂量增加而明显增加，而Hp^ 细胞凋亡率增加不明显。结论小鼠骨髓组织中可以检测到结合珠蛋白的存在，并且在辐射后有明显的增加，骨髓细胞中Hp阴、阳性细胞的周期分布和凋亡存在明显的差异。推测Hp可能参与辐射诱导骨髓损伤的修复过程。

Research on the expression of haptoglobin in bone marrow of irradiated mice

Objective To study the expression and distribution of the haptoglobin in the bone marrow of irradiated mice and explore the relationship between haptoglobin and cell cycle or apoptosis. Methods RT-PCR and Western blot were adopted to respectively detect the changes of the haptoglobin at mRNA and protein levels. Flow cytometry (FCM) was applied to analyze the relation between cytoplasmic haptoglobin existence in bone marrow and cell cycle or apoptosis. Results The results showed that increased levels of haptoglobin mRNA and protein were present after irradiation. Results of FCM indicated there was difference in cell cycle between the cells with and without haptoglobin protein. Conclusion Haptoglobin might be involved in the repair process of radiation injury.;