| 远缘链球菌细胞分裂蛋白的表达、纯化和抗体的制备 |
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| 引用本文： | 金涛,吴补领,苏凌云,高杰.远缘链球菌细胞分裂蛋白的表达、纯化和抗体的制备[J].陕西医学杂志,2005,34(5):518-520. |
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| 作者姓名： | 金涛 吴补领 苏凌云 高杰 |
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| 作者单位： | 第四军医大学口腔医学院,西安,710032 |
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| 基金项目： | 第四军医大学创新工程资助项目(NoCX02A012) |
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| 摘 要： | 目的表达与纯化远缘链球菌克隆细胞分裂蛋白(ftsK),制备多克隆抗体并鉴定。方法利用IPTG诱导ftsK在大肠杆菌中表达,随后以再生Ni-NTA柱纯化蛋白。纯化后的蛋白多次注射于新西兰大白兔中,获取多克隆抗血清,ELISA法检测抗体效价,Westernblot法检测抗体特异性。结果获得了纯度较高的远缘链球菌ftsK蛋白及其抗体,多抗效价达到1∶10000,特异性较好。结论成功表达了远缘链球菌ftsK蛋白,获得了效价较高、特异性较强的多克隆抗体,为进一步研究ftsK蛋白功能获取了良好的工具。
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| 关 键 词： | 细胞周期蛋白类/代谢 @多克隆抗体 动物 实验 兔 |
Expression of streptococcus sobrinus's fts K protein and preparation of polyclonal antibody |
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| Jin Tao Wu Buling Su Lingyun et alCollege of Stomatology,the Fourth Military MedicalUniversity.Expression of streptococcus sobrinus''''s fts K protein and preparation of polyclonal antibody[J].Shaanxi Medical Journal,2005,34(5):518-520. |
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| Authors: | Jin Tao Wu Buling Su Lingyun College of Stomatology the Fourth Military MedicalUniversity |
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| Institution: | Xi'an 710032 |
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| Abstract: | Objective: To express the streptococcus sobrinus's fts K protein and prepare polyclonal antibody against the protein. Methods: IPTG was used to induce the expression of fts K protein, Ni-NTA column was used to purify the protein. Polyclonal antibody was prepared by immunizing rabbits with this protein. The expressed protein was identified by ELISA and western blot. Results: The purified streptococcus sobrinus's fts K protein was obtained, the title of prepared polyclonal antibody is 1∶10000, and the antibody's specificity is very high. Conclusion: The purified streptococcus sobrinus's fts K protein and polyclonal antibody was successfully obtained. The expression of fts K in E coli provided the basic material for studying the role of fts K, and polyclonal antibody is a good tool for the function research of fts K. |
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| Keywords: | Cell cycle protein/metabolism @Polyclonal antibody Animals laboratory Rabbbits |
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