高浓度臭氧暴露法建立大鼠哮喘模型

目的评价用卵蛋白（OVA）叠加高浓度臭氧（O3）暴露方法建立大鼠严重支气管哮喘模型的方法。方法随机数表法将30只sD大鼠分为正常组、卵蛋白激发组（OVA组）及卵蛋白叠加臭氧暴露组（OVA／O3）。采用OVA腹腔注射致敏，结合OVA持续雾化吸入和臭氧暴露方法，建立重度哮喘模型。观察大鼠哮喘发作期间症状，苏木素-伊红（HE）染色观察大鼠肺组织形态变化及炎症细胞浸润情况。采用免疫组化方法检测NF—κBp65亚基表达变化。结果以OVA激发期间OVA／O3大鼠出现最严重哮喘症状；HE染色结果显示OVA组及OVA／O3组有明显嗜酸性粒细胞浸润，并伴有支气管上皮细胞脱落，OVA／O3现象最为明显。p65在肺组织中的表达以平均光密度（IODmean）计分别为：正常组（0．0146±0．0013），OVA组（0．2502±0．0009），OVA／03（0．4240±0．0011），两模型组显著高于正常组，差异有统计学意义（P〈0．01），OVA／O3组亦显著高于OVA组（P〈0．01）。结论用臭氧暴露结合OVA致敏方法成功建立重度哮喘大鼠模型。

Establishment of an asthma model usign a high concentration of ozone

Objective To establish a severe asthma model in ovalbumin OVA-sensitized Sprague Dawley rat. Methods Thirty Sprague Dawley rats were randomly divided into physiological saline control group, OVA group and OVA/O3 group. The severe asthma model was established by sensitizating the rats with intraperitoneal injection of OVA,followed by repeated inhalation of OVA and ozone.Asthmatic symptoms were observed.Lung tissue sections were stained with hematoxylin and eosin （HE） for the examination of infiltration of inflammatory cells. Immunohistochemistry was applied to examine the expression of p65 subunit. Results Asthmatic symptoms were more severe in the OVA/O3 group than the OVA group and control group. Extensive inflammatory cells （eosinophils,neutrophits, lymphocytes） infiltration around the bronchi and epithelial shedding was observed in the OVA/O3 group.The mean IOD of p65 expression in OVA/O3 group, OVA group and control was （0.0146±0.0013）,（0.2502±0.0009） and （0.4240±0.0011）,respectively,and the difference was significant （P〈0.01） as compared with the OVA group. Conclusion An animal model of severe asthma was successfully established by exposuring OVA-sensitized Sprague Dawley rats to ozone.