| 分化抑制因子Idl对骨肉瘤细胞增殖的影响 |
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| 引用本文： | 陈燕,杨光,杨祖华. 分化抑制因子Idl对骨肉瘤细胞增殖的影响[J]. 广东寄生虫学会年报, 2014, 0(1): 71-74 |
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| 作者姓名： | 陈燕 杨光 杨祖华 |
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| 作者单位： | [1]合肥市第三人民医院骨科,安徽合肥230022 [2]中山大学肿瘤防治中心华南肿瘤学国家重点实验室,广东广州510060 |
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| 摘 要： | 目的探讨分化抑制因子Idl对骨肉瘤细胞增殖的影响及可能的途径。方法采用四甲基偶氮唑蓝（MTY）法检测Idl表达调节后MG．63细胞增殖变化情况；采用流式细胞术检测Idl下调表达后，MG．63细胞凋亡及细胞周期变化情况；Westemblotting技术检测Idl下调表达后对p-AKT水平的影响。结果本实验利用RNA干扰技术下调骨肉瘤细胞系Idl表达后，可以明显抑制MG．63细胞的增殖，p-AKT的水平也显示下降，流式细胞术证明下调后Idl的水平可以一定程度地调节细胞凋亡水平，而对细胞周期无明显影响，另外进一步构建了Idl的真核表达质粒，转染Idl过表达质粒后。并未见骨肉瘤细胞明显地增殖加速。结论分化抑制因子Idl可能通过影响AKT信号通路进而影响了骨肉瘤细胞的增殖。
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| 关 键 词： | 骨肉瘤 细胞系 MG 63 Idl 增殖 |
Effect of DNA-binding protein inhibitor Idl on the proliferation of osteosarcoma cells |
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| CHEN Yan,YANG Guang,YANG Zu-hua. Effect of DNA-binding protein inhibitor Idl on the proliferation of osteosarcoma cells[J]. Journal of Tropical Medicine, 2014, 0(1): 71-74 |
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| Authors: | CHEN Yan YANG Guang YANG Zu-hua |
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| Affiliation: | 1.Department of Orthopedics, the Third People＇s Hospital of Hefei City, Anhui,Hefei 230022; 2.State Key Laboratory of Oncology in South China, Cancer Center, Sun Yat-sen University, Guangdong, Guangzhou 510060, China) |
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| Abstract: | Objective To study the effect of DNA-binding protein inhibitor Idl on the growth of osteosarcoma cells. Methods MTT assay was used to evaluate the proliferation of osteosarcoma MG-63 ceils. Flow cytometry was used to investigate the cell cycle and apoptosis. Western blotting was performed to detect the level of p-AKT. Results RNAi silencing of Idl was found to induce apoptosis and exert a growth inhibitory effect on MG-63 cells. There was no obvious effect on the change of cell cycle. The level of p-AKT was found to be downregulated by Idl RNAi. However, overexpressionion of Idl showed no effect on the proliferation of MG-63 cells. Conclusion Idl was found to be involved in the proliferation of osteosarcoma cells. |
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| Keywords: | osteosarcoma cell line MG-63 Idl proliferation |
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