硫酸吲哚酚对THP-1源性泡沫细胞ATP结合转运子A1和G1表达的调控

目的观察硫酸吲哚酚（is）是否调节巨噬泡沫细胞脂滴的蓄积，以及对巨噬泡沫细胞ATP结合转运子Al（ABCAl）和ATP结合转运子G1（ABCGl）表达的影响。方法体外佛波酯（PMA）诱导人源单核细胞系THP．1分化为巨噬细胞，随后加入氧化低密度脂蛋白（OX．LDL）刺激下进一步转变为泡沫细胞。巨噬细胞加入不同浓度的IS以及OX．LDL共同作用24h，油红O染色法观察细胞内脂滴的含量，RT．PCR法检测ABCAl和ABCGlmRNA水平．Western blotting法检测ABCAl和ABCGl蛋白表达。结果Is干预可明显促进细胞内脂滴蓄积。下调单核／巨噬细胞泡沫化过程中ABCAl和ABCGlmRNA和蛋白的表达，且此作用呈浓度依赖性。结论IS可通过下调ABCAl和ABCGlmRNA和蛋白的表达，增加细胞内脂滴蓄积，从而促进动脉粥样硬化的发生发展。

Regulation of indoxyl sulfate on expression of ATP-binding cassette transporter A1/G1 in THP-1 derived foam cells

Objective To investigate the regulation of indoxyl sulfate on the expression of ATP-binding cassette transporter A 1/G 1 （ABCA1/ABCG1 ） during the foam cell foramtion. Methods The human monocytic leukemia cell line （THP- 1 ） was chosen for our study. The differentiation of THP-1 cells into macrophages was induced by using phorbol myristate acetate （PMA）. Macrophages were then incubated with oxidized LDL （ox-LDL） to generate foam cells. Indoxyl sulfate of different concentrations were added at different time points during foam cell formations. The ABCAI/ABCG1 protein and mRNA levels were detected by Western blotting and RT-PCR. The effect of variance of cholesterol content was measured by zymochemistry via-fluorospectrophotometer. Results Indoxyl sulfate increased the content of lipid droplet in foam cells, and reduced the efflux of intracellular cholesterol significantly. Indoxyl sulfate reduced ABCA1 protein mass and mRNA levels in dosedependent manner. The changes of ABCG1 protein and mRNA level were the same as ABCA1. Conclusion Indoxyl sulfate interfere atherosclerosis by down-regulating the expression of ABCA1 and ABCGI.