| HLA—DR位点的PCR—SSP基因分型 |
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| 引用本文： | 徐鸿绪,曾文涛,陈连周,王晓波.HLA—DR位点的PCR—SSP基因分型[J].中国优生与遗传杂志,2001,9(4):9-10. |
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| 作者姓名： | 徐鸿绪 曾文涛 陈连周 王晓波 |
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| 作者单位： | 中山医科大学附属第一医院外科实验室,广州,510080 |
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| 摘 要： | 目的：探讨适用于肾移植供体HLA-DR的快速基因分型方法。方法：自行设计合成引物建立顺序特异性引物聚合酶链反应技术，并对110例肾移植供体作HLA-DR基因分型。结果：DNA提取方法可以满足PCR-SSP分型方法对DNA模板的要求。全部操作耗时120min,110例标本均分型成功。基因频率范畴在0.0045-0.1227之间，以DR4、DR15和DR9占多数。结论：PCR-SSP法作HLA-DR分型具有准确，简便，快速等优点。适合在临床进行推广应用。
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| 关 键 词： | 肾移植 供体 HLA－DR位点 PCR－SSP 基因分型 |
HLA- DR locus genotyping by PCR- SSP methods |
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| Xu Hongxu,Zeng Wentao,Chen Lianzhou,et al..HLA- DR locus genotyping by PCR- SSP methods[J].Chinese Journal of Birth Health & Heredity,2001,9(4):9-10. |
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| Authors: | Xu Hongxu Zeng Wentao Chen Lianzhou |
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| Abstract: | Objective:To explore a new and practical whole blood DNA genotyping technique of HLA-DR locus in the donors of renal transplantation.Methods:Design and synthesize primers to establish the polymerase chain reaction sequence-specific primer(PCR-SSP)method based on DNA extraction for HLA-DR locus genotyping.Results:The DNA extraction method was satisfaction of study requirement for PCR-SSP.110 samples of whole blood were successfully typed by means of PCR-SSP and the procedure takes about 2 hours.The gene frequency of the groups is between 0.0045-0.1227.DR4,DR15 and DR9 have a larger proportion than others.Conclusions:The PCR-SSP typing is a simple,rapid,reliable and accurate technique.It is suitable for clinical application and expansion. |
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| Keywords: | HLA PCR-SSP Genotyping Transplantation |
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